Overview

  • Product name

  • Description

    Chicken polyclonal to SIRT6
  • Host species

    Chicken
  • Specificity

    ab105391 is predicted to not cross-react with other Sirtuin family members. Multiple isoforms of SIRT6 exist.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    17 amino acid synthetic peptide near the C-terminus of Human SIRT6 (EAW69254).

  • Positive control

    • HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab105391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 39 kDa.

Target

  • Function

    NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate.
  • Sequence similarities

    Belongs to the sirtuin family. Class IV subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • Cellular localization

    Nucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2810449N18Rik antibody
    • AI043036 antibody
    • Mono ADP ribosyltransferase sirtuin 6 antibody
    • NAD-dependent protein deacetylase sirtuin-6 antibody
    • Regulatory protein SIR2 homolog 6 antibody
    • Regulatory protein SIR2 homolog antibody
    • SIR2 like 6 antibody
    • SIR2 like protein 6 antibody
    • Sir2 related protein type 6 antibody
    • SIR2-like protein 6 antibody
    • SIR2L6 antibody
    • SIR6_HUMAN antibody
    • SIRT 6 antibody
    • SIRT6 antibody
    • Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) antibody
    • Sirtuin 6 antibody
    • Sirtuin type 6 antibody
    • Sirtuin6 antibody
    see all

Images

  • Lane 1 : Anti-SIRT6 antibody (ab105391) at 0.5 µg/ml
    Lane 2 : Anti-SIRT6 antibody (ab105391) at 1 µg/ml

    All lanes : HeLa cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 39 kDa

References

This product has been referenced in:

  • Zhang L  et al. Reprogramming metabolism by targeting sirtuin 6 attenuates retinal degeneration. J Clin Invest 126:4659-4673 (2016). Read more (PubMed: 27841758) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us. SIRT6 The human, mouse and rat proteins are homologous in N terminal and central region of protein. So the antibodies available to these regions will most likely cross-react. ab62739, cross reacts with human, mouse and rat. Not yet tested in Immunohistochemistry.  ab105391, cross reacts with human, mouse and rat. Not yet tested in Immunohistochemistry. These antibodies are not yet tested in Immunohistochemistry. However this does not mean that these are not suitable for IHC it only mean we do not have tested data to confirm their use. If you are interested in testing one of these in IHC then let me know I will send you the Abreview discount code;  which states we will offer a next free antibody in exchange of an antibody review.    SIRT1 ab32441 and ab32424 These antibodies are tested in IHC so are fully guaranteed to work in IHC. The mouse and rat species is predicted which is based on sequence homology of immunogen between these species. If you are interested in using these antibodies in mouse and rat then let me know I will provide you the Abreview discount code which will give you your next purchase of an antibody free of charge. ab75435 and ab110304 does cross react with human, mouse and rat however not tested in IHC therefore these are eligible for Abreview discount programme.   SIRT2 ab115681 is tested in human and mouse and in IHC-P. ab67299 and ab75436 are tested for cross reactivity in human, mouse and rat however not tested in IHC. These products are also eligible for Abreview discount programme. I also have attached a spreadsheet of all anti SIRT6, SIRT2 and SIRT1 antibodies with Immunogen sequence. Please have a look and decide which one best suits you. For information about publications and images please check the datasheets of individual antibodies. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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