Overview

  • Product name

  • Description

    Rabbit polyclonal to SIRT6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human SIRT6 aa 50-100. The exact sequence is proprietary. (NP_057623.1).
    Database link: Q8N6T7

  • Positive control

    • IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab245348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate.
    • Sequence similarities

      Belongs to the sirtuin family. Class IV subfamily.
      Contains 1 deacetylase sirtuin-type domain.
    • Cellular localization

      Nucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions.
    • Information by UniProt
    • Database links

    • Alternative names

      • 2810449N18Rik antibody
      • AI043036 antibody
      • Mono ADP ribosyltransferase sirtuin 6 antibody
      • NAD-dependent protein deacetylase sirtuin-6 antibody
      • Regulatory protein SIR2 homolog 6 antibody
      • Regulatory protein SIR2 homolog antibody
      • SIR2 like 6 antibody
      • SIR2 like protein 6 antibody
      • Sir2 related protein type 6 antibody
      • SIR2-like protein 6 antibody
      • SIR2L6 antibody
      • SIR6_HUMAN antibody
      • SIRT 6 antibody
      • SIRT6 antibody
      • Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) antibody
      • Sirtuin 6 antibody
      • Sirtuin type 6 antibody
      • Sirtuin6 antibody
      see all

    Images

    • SIRT6 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245348 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different Anti-SIRT6 antibody at 1 µg/ml.

      Lane 1: ab245348 IP in HeLa whole cell lysate.
      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Detection: Chemiluminescence with exposure time of 30 seconds.

    References

    ab245348 has not yet been referenced specifically in any publications.

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