Key features and details
- Rabbit polyclonal to SIRT6
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SIRT6 antibody
See all SIRT6 primary antibodies
DescriptionRabbit polyclonal to SIRT6
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human SIRT6 aa 50-100. The exact sequence is proprietary. (NP_057623.1).
Database link: Q8N6T7
- IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245348 was affinity purified using an epitope specific to SIRT6 immobilized on solid support.
- Epigenetics and Nuclear Signaling
- Chromatin Modifying Enzymes
- Class III / Sir2 class
Our Abpromise guarantee covers the use of ab245348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
FunctionNAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate.
Sequence similaritiesBelongs to the sirtuin family. Class IV subfamily.
Contains 1 deacetylase sirtuin-type domain.
Cellular localizationNucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions.
- Information by UniProt
- 2810449N18Rik antibody
- AI043036 antibody
- Mono ADP ribosyltransferase sirtuin 6 antibody
SIRT6 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245348 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different Anti-SIRT6 antibody at 1 µg/ml.
Lane 1: ab245348 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245348 has not yet been referenced specifically in any publications.