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Thanks for the further information. I have one final question: using the histone extraction protocol, it says to acid extract in 0.2 N HCl; is that 0.2 N added to the extraction buffer, or is it just simply acid diluted in water?
Asked on Apr 11 2012
Thank you for your question.
I hope I can clarify the protocol:
Step 5. is a repetition of step 4.: this provides you the pellet for furtheruse.
Step 6. of the protocol: Re-suspend the pellet in 0.2 N HCl at a density of 4x107 nuclei per ml.
And indeed Step 6. means that you will just use "acid diluted in water" and not the TEB plus 0.2 N HCl.
I have forwarded this to our protocolteam as well and hope they will rephrase it a bit clearer in the future.
Wish you all the best for your experiments.
Answered on Apr 11 2012