Validated using a knockout cell line
Recombinant
RabMAb

Anti-SIRT6 antibody [EPR5079(N)] (ab176345)

Overview

  • Product name
    Anti-SIRT6 antibody [EPR5079(N)]
    See all SIRT6 primary antibodies
  • Description
    Rabbit monoclonal [EPR5079(N)] to SIRT6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SIRT6 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8N6T7

  • Positive control
    • K562, Jurkat, 293T, HepG2 and HeLa cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab176345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 39 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate.
    • Sequence similarities
      Belongs to the sirtuin family. Class IV subfamily.
      Contains 1 deacetylase sirtuin-type domain.
    • Cellular localization
      Nucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2810449N18Rik antibody
      • AI043036 antibody
      • Mono ADP ribosyltransferase sirtuin 6 antibody
      • NAD-dependent protein deacetylase sirtuin-6 antibody
      • Regulatory protein SIR2 homolog 6 antibody
      • Regulatory protein SIR2 homolog antibody
      • SIR2 like 6 antibody
      • SIR2 like protein 6 antibody
      • Sir2 related protein type 6 antibody
      • SIR2-like protein 6 antibody
      • SIR2L6 antibody
      • SIR6_HUMAN antibody
      • SIRT 6 antibody
      • SIRT6 antibody
      • Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) antibody
      • Sirtuin 6 antibody
      • Sirtuin type 6 antibody
      • Sirtuin6 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)              
      Lane 2: SIRT6 knockout  HAP1 whole cell lysate (20 µg)
      Lane 3: HepG2 whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - ab176345 observed at 45 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab176345 was shown to specifically recognize SIRT6 in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when SIRT6 knockout samples were examined. Wild-type and SIRT6 knockout samples were subjected to SDS-PAGE.  Ab176345 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/30,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-SIRT6 antibody [EPR5079(N)] (ab176345) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 39 kDa

    References

    This product has been referenced in:
    • Jin Y  et al. Double deletion of tetraspanins CD9 and CD81 in mice leads to a syndrome resembling accelerated aging. Sci Rep 8:5145 (2018). WB . Read more (PubMed: 29572511) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (melanoma)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    500000 cells
    Specification
    melanoma
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 23 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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