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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SIRT6 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q8N6T7
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab176345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 39 kDa.|
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: SIRT6 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab176345 observed at 45 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab176345 was shown to specifically recognize SIRT6 in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when SIRT6 knockout samples were examined. Wild-type and SIRT6 knockout samples were subjected to SDS-PAGE. Ab176345 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/30,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"