Product nameAnti-SIRT6 antibody [OTI1G3]
See all SIRT6 primary antibodies
DescriptionMouse monoclonal [OTI1G3] to SIRT6
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Dog, Human, African green monkey
Recombinant fragment corresponding to Human SIRT6 aa 88-352. Produced in E.coli (NP_057623).
SARPTQTHMALVQLERVGLLRFLVSQNVDGLHVRSGFPRDKLAELHGNMF VEECAKCKTQYVRDTVVGTMGLKATGRLCTVAKARGLRACRGELRDTILD WEDSLPDRDLALADEASRNADLSITLGTSLQIRPSGNLPLATKRRGGRLV IVNLQPTKHDRHADLRIHGYVDEVMTRLMKHLGLEIPAWDGPRVLERALP PLPRPPTPKLEPKEESPTRINGSIPAGPKQEPCAQHNGSEPASPKRERPT SPAPHRPPKRVKAKA
Database link: Q8N6T7
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY SIRT6 cDNA; HepG2, HeLa, COS-7, Jurkat, MDCK and MCF7 cell extracts. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY SIRT6.
Clone OTI1G3 (formerly 1G3).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
Purification notesPurified from cell culture supernatant by affinity chromatography.
- Epigenetics and Nuclear Signaling
- Chromatin Modifying Enzymes
- Class III / Sir2 class
Our Abpromise guarantee covers the use of ab119007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 39 kDa.|
FunctionNAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate.
Sequence similaritiesBelongs to the sirtuin family. Class IV subfamily.
Contains 1 deacetylase sirtuin-type domain.
Cellular localizationNucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions.
- Information by UniProt
- 2810449N18Rik antibody
- AI043036 antibody
- Mono ADP ribosyltransferase sirtuin 6 antibody
All lanes : Anti-SIRT6 antibody [OTI1G3] (ab119007) at 1/200 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : SVT2 (Mouse) cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (canine kidney cell line) cell extract
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 39 kDa
All lanes : Anti-SIRT6 antibody [OTI1G3] (ab119007) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY SIRT6 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 39 kDa
COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY SIRT6 stained for SIRT6 (green) using ab119007 at 1/100 dilution in ICC/IF.
ab119007 has not yet been referenced specifically in any publications.