Key features and details
- Mouse monoclonal [1E2B2] to SIRT7
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-SIRT7 antibody [1E2B2]
See all SIRT7 primary antibodies
DescriptionMouse monoclonal [1E2B2] to SIRT7
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human SIRT7 aa 1-105. Expressed in E. coli.
Database link: Q9NRC8
- WB: SIRT7 (amino acids: 1-105)-hIgGFc transfected HEK-293 cell lysate; Human recombinant SIRT7 (amino acids: 1-105) protein. Flow Cytometry: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
- Epigenetics and Nuclear Signaling
- Chromatin Modifying Enzymes
- Class III / Sir2 class
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Purine and pyrimidine synthesis
Our Abpromise guarantee covers the use of ab234088 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500.|
|Flow Cyt||1/200 - 1/400.|
FunctionNAD-dependent protein deacetylase that specifically mediates deacetylation of histone H3 at 'Lys-18' (H3K18Ac). In contrast to other histone deacetylases, displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression. H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors. SIRT7 thereby acts as a transcription repressor. Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells. These data suggest that SIRT7 may play a key role in oncogenic transformation by suppresses expression of tumor suppressor genes by locus-specific deacetylation of H3K18Ac at promoter regions. Also required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis: promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. May also deacetylate p53/TP53 and promotes cell survival, however such data need additional confirmation.
Sequence similaritiesBelongs to the sirtuin family. Class IV subfamily.
Contains 1 deacetylase sirtuin-type domain.
modificationsPhosphorylated during mitosis.
Cellular localizationCytoplasm. Nucleus, nucleolus. Located close to the nuclear membrane when in the cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis.
- Information by UniProt
- NAD dependent deacetylase sirtuin 7 antibody
- NAD dependent protein deacetylase sirtuin 7 antibody
- NAD-dependent protein deacetylase sirtuin-7 antibody
Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, labeling SIRT7 with ab234088 at 1/200 dilution (green) compared to a negative control (red).
All lanes : Anti-SIRT7 antibody [1E2B2] (ab234088) at 1/100 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : SIRT7 (amino acids: 1-105)-hIgGFc transfected HEK-293 cell lysate
Anti-SIRT7 antibody [1E2B2] (ab234088) at 1/100 dilution + Human recombinant SIRT7 (amino acids: 1-105) protein
Expected recombinant MW is 38 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234088 has not yet been referenced specifically in any publications.