Product nameAnti-SIRT7 antibody
See all SIRT7 primary antibodies
DescriptionRabbit polyclonal to SIRT7
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
- Extracts of HEK-293T cells expressing human SIRT7, HEK-293T cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Chromatin Modifying Enzymes
- Class III / Sir2 class
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Purine and pyrimidine synthesis
Our Abpromise guarantee covers the use of ab62748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2-4 ug.
WB: Use at a concentration of 1 - 2 ug/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionNAD-dependent protein deacetylase that specifically mediates deacetylation of histone H3 at 'Lys-18' (H3K18Ac). In contrast to other histone deacetylases, displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression. H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors. SIRT7 thereby acts as a transcription repressor. Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells. These data suggest that SIRT7 may play a key role in oncogenic transformation by suppresses expression of tumor suppressor genes by locus-specific deacetylation of H3K18Ac at promoter regions. Also required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis: promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. May also deacetylate p53/TP53 and promotes cell survival, however such data need additional confirmation.
Sequence similaritiesBelongs to the sirtuin family. Class IV subfamily.
Contains 1 deacetylase sirtuin-type domain.
modificationsPhosphorylated during mitosis.
Cellular localizationCytoplasm. Nucleus, nucleolus. Located close to the nuclear membrane when in the cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis.
- Information by UniProt
- NAD dependent deacetylase sirtuin 7 antibody
- NAD dependent protein deacetylase sirtuin 7 antibody
- NAD-dependent protein deacetylase sirtuin-7 antibody
Human HEK-293T cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.2% Triton-X-100. Cells were stained with 2.5 µg/mL Anti-SIRT7 (ab62748) and developed with Goat Anti-Rabbit IgG, Cy3 conjugate (A), or stained with DAPI (B).
ab62748 was used to immunoprecipitate SIRT7 from HEK-293T cells expressing recombinant human SIRT7-V5. Detection antibody: Monoclonal Anti-V5 antibody. Lanes 1: 2 µg Antibody 2: 4 µg Antibody 3: Negative control (without primary antibody)
This product has been referenced in:
- Deng Z et al. Sirtuin 7 promotes colorectal carcinoma proliferation and invasion through the inhibition of E-cadherin. Exp Ther Med 15:2333-2342 (2018). Read more (PubMed: 29467843) »
- Yan WW et al. Arginine methylation of SIRT7 couples glucose sensing with mitochondria biogenesis. EMBO Rep 19:N/A (2018). Read more (PubMed: 30420520) »