Product nameAnti-SIRT7 antibody
See all SIRT7 primary antibodies
DescriptionRabbit polyclonal to SIRT7
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig
- Kidney tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein G purified
- Epigenetics and Nuclear Signaling
- Chromatin Modifying Enzymes
- Class III / Sir2 class
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Purine and pyrimidine synthesis
Our Abpromise guarantee covers the use of ab78977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 45 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionNAD-dependent protein deacetylase that specifically mediates deacetylation of histone H3 at 'Lys-18' (H3K18Ac). In contrast to other histone deacetylases, displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression. H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors. SIRT7 thereby acts as a transcription repressor. Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells. These data suggest that SIRT7 may play a key role in oncogenic transformation by suppresses expression of tumor suppressor genes by locus-specific deacetylation of H3K18Ac at promoter regions. Also required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis: promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. May also deacetylate p53/TP53 and promotes cell survival, however such data need additional confirmation.
Sequence similaritiesBelongs to the sirtuin family. Class IV subfamily.
Contains 1 deacetylase sirtuin-type domain.
modificationsPhosphorylated during mitosis.
Cellular localizationCytoplasm. Nucleus, nucleolus. Located close to the nuclear membrane when in the cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis.
- Information by UniProt
- NAD dependent deacetylase sirtuin 7 antibody
- NAD dependent protein deacetylase sirtuin 7 antibody
- NAD-dependent protein deacetylase sirtuin-7 antibody
ab78977 staining SIRT7 in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% triton X-100 and blocked with 3% BSA for 2 hours at 25°C; antigen retrieval was by heat mediation in 0.1M citrate buffer containing 0.05% triton X-100. Samples were incubated with primary antibody (1/1000 in 1% BSA containing 0.1% triton X-100) for 16 hours at 4°C. A Cy3 ®-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
ab78977 at 5µg/ml staining SIRT7 in kidney tissue.
This product has been referenced in:
- Wang X et al. microRNA-20b contributes to high glucose-induced podocyte apoptosis by targeting SIRT7. Mol Med Rep 16:5667-5674 (2017). Read more (PubMed: 28849008) »
- McGlynn LM et al. SIRT3 & SIRT7: Potential Novel Biomarkers for Determining Outcome in Pancreatic Cancer Patients. PLoS One 10:e0131344 (2015). IHC ; Human . Read more (PubMed: 26121130) »