Product nameSIVA overexpression 293T lysate (whole cell)
See all SIVA lysates
DescriptionSIVA overexpression lysate
ab94199 is a 293T cell transfected lysate in which Human SIVA has been transiently over-expressed using a pCMV-SIVA plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
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BackgroundFunction: Induces CD27-mediated apoptosis. Inhibits BCL2L1 isoform Bcl-x(L) anti-apoptotic activity. Inhibits activation of NF-kappa-B and promotes T-cell receptor-mediated apoptosis. Tissue specificity: Ubiquitous. Mostly expressed in thymus, testis, ovary, prostate, small intestine and spleen and less in colon. PTM: Phosphorylated by ABL2/ARG in response to oxidative stress.
Our Abpromise guarantee covers the use of ab94199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94199 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-SIVA antibody (ab67620) at 1/500 dilution
Lane 1 :
SIVA overexpression 293T lysate (whole cell) (ab94199)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94199 has not yet been referenced specifically in any publications.