Recombinant Anti-SIX1 antibody [1593] - BSA and Azide free (ab255754)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [1593] to SIX1 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-SIX1 antibody [1593] - BSA and Azide free
See all SIX1 primary antibodies -
Description
Mouse monoclonal [1593] to SIX1 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A-204 whole cell lysate. IHC-P: Human, mouse and rat skeletal muscle tissue.
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General notes
ab255754 is the carrier-free version of ab243247.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1593 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry reagents
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab255754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.459 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
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IHC-P |
Use a concentration of 2.295 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.459 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). |
IHC-P
Use a concentration of 2.295 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
May be involved in limb tendon and ligament development. -
Tissue specificity
Specifically expressed in skeletal muscle. -
Involvement in disease
Defects in SIX1 are the cause of deafness autosomal dominant type 23 (DFNA23) [MIM:605192]. A form of non-syndromic deafness characterized by prelingual, bilateral, symmetric hearing loss with a conductive component present in some but not all patients.
Defects in SIX1 are the cause of branchiootic syndrome type 3 (BOS3) [MIM:608389]. BOS3 is a syndrome characterized by usually bilateral branchial cleft fistulas or cysts, sensorineural and/or conductive hearing loss, pre-auricular pits, and structural defects of the outer, middle or inner ear. Otic defects include malformed and hypoplastic pinnae, a narrowed external ear canal, bulbous internal auditory canal, stapes fixation, malformed and hypoplastic cochlea. Branchial and otic anomalies are as those seen in individuals with the branchiootorenal syndrome. However, renal anomalies are absent in branchiootic syndrome patients.
Note=Defects in SIX1 could be a cause of branchiootorenal syndrome (BOR). BOR is an autosomal dominant disorder manifested by various combinations of preauricular pits, branchial fistulae or cysts, lacrimal duct stenosis, hearing loss, structural defects of the outer, middle, or inner ear, and renal dysplasia. Associated defects include asthenic habitus, long narrow facies, constricted palate, deep overbite, and myopia. Hearing loss may be due to mondini type cochlear defect and stapes fixation. Penetrance of BOR syndrome is high, although expressivity can be extremely variable. -
Sequence similarities
Belongs to the SIX/Sine oculis homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6495 Human
- Entrez Gene: 20471 Mouse
- Entrez Gene: 114634 Rat
- Omim: 601205 Human
- SwissProt: Q15475 Human
- SwissProt: Q62231 Mouse
- Unigene: 713114 Human
- Unigene: 4645 Mouse
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Alternative names
- BOS3 antibody
- DFNA23 antibody
- Homeobox protein SIX1 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling SIX1 with ab243247 at 2.295µg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on human skeletal muscle tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab243247).
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Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling SIX1 with ab243247 at 2.295µg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on mouse skeletal muscle tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).This image was produced using the same antibody clone but in a different formulation ab243247, PBS, sodium azide, glycerol and BSA.
This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab243247).
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Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling SIX1 with ab243247 at 2.295µg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on rat skeletal muscle tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).This image was produced using the same antibody clone but in a different formulation ab243247, PBS, sodium azide, glycerol and BSA.
This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab243247).
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All lanes : Anti-SIX1 antibody [1593] (ab243247) at 0.459 µg/ml
Lane 1 : A-204 (human muscle rhabdomyosarcoma), whole cell lysate
Lane 2 : Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 32 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab243247).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab255754 has not yet been referenced specifically in any publications.