Product nameAnti-SIX1 antibody
See all SIX1 primary antibodies
DescriptionRabbit polyclonal to SIX1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Three synthetic peptides (human) conjugated to KLH
- An induced culture of E. coli
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay be involved in limb tendon and ligament development.
Tissue specificitySpecifically expressed in skeletal muscle.
Involvement in diseaseDefects in SIX1 are the cause of deafness autosomal dominant type 23 (DFNA23) [MIM:605192]. A form of non-syndromic deafness characterized by prelingual, bilateral, symmetric hearing loss with a conductive component present in some but not all patients.
Defects in SIX1 are the cause of branchiootic syndrome type 3 (BOS3) [MIM:608389]. BOS3 is a syndrome characterized by usually bilateral branchial cleft fistulas or cysts, sensorineural and/or conductive hearing loss, pre-auricular pits, and structural defects of the outer, middle or inner ear. Otic defects include malformed and hypoplastic pinnae, a narrowed external ear canal, bulbous internal auditory canal, stapes fixation, malformed and hypoplastic cochlea. Branchial and otic anomalies are as those seen in individuals with the branchiootorenal syndrome. However, renal anomalies are absent in branchiootic syndrome patients.
Note=Defects in SIX1 could be a cause of branchiootorenal syndrome (BOR). BOR is an autosomal dominant disorder manifested by various combinations of preauricular pits, branchial fistulae or cysts, lacrimal duct stenosis, hearing loss, structural defects of the outer, middle, or inner ear, and renal dysplasia. Associated defects include asthenic habitus, long narrow facies, constricted palate, deep overbite, and myopia. Hearing loss may be due to mondini type cochlear defect and stapes fixation. Penetrance of BOR syndrome is high, although expressivity can be extremely variable.
Sequence similaritiesBelongs to the SIX/Sine oculis homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- BOS3 antibody
- DFNA23 antibody
- Homeobox protein SIX1 antibody
All lanes : Anti-SIX1 antibody (ab86028) at 1/1000 dilution
Lane 1 : An un-induced culture of E. coli
Lane 2 : An induced culture of E. coli
Lysates/proteins at 1/5000 dilution per lane.
All lanes : Anti-Rabbit IgG HRP at 1/1000 dilution
Predicted band size: 32 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 60 minutes
Gel concentration 10%