For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    six1-antibody-cl0185-ab236761.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Transcription Cancer susceptibility Proto-oncogenes
Share by email

Anti-SIX1 antibody [CL0185] (ab236761)

  • Datasheet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-SIX1 antibody [CL0185] (ab236761)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [CL0185] (ab236761)
  • Immunocytochemistry/ Immunofluorescence - Anti-SIX1 antibody [CL0185] (ab236761)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [CL0185] (ab236761)
  • Western blot - Anti-SIX1 antibody [CL0185] (ab236761)

Key features and details

  • Mouse monoclonal [CL0185] to SIX1
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

You may also be interested in

Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-SIX1 antibody [CL0185]
    See all SIX1 primary antibodies
  • Description

    Mouse monoclonal [CL0185] to SIX1
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Gorilla
  • Immunogen

    Recombinant fragment corresponding to Human SIX1 aa 130-270.
    Sequence:

    CFKEKSRGVL REWYAHNPYP SPREKRELAE ATGLTTTQVS NWFKNRRQRD RAAEAKEREN TENNNSSSNK QNQLSPLEGG KPLMSSSEEE FSPPQSPDQN SVLLLQGNMG HARSSNYSLP GLTASQPSHG LQTHQHQLQD S


    Database link: Q15475
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • ICC/IF: RH-30 cells. WB: RH-30 cell lysate. IHC-P: Human striated muscle tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    CL0185
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Differentiation

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Related Products

    • Recombinant Human SIX1 protein (denatured) (ab134521)

Applications

Our Abpromise guarantee covers the use of ab236761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500.

HIER pH 6

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.
ICC/IF Use a concentration of 2 - 10 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Function

    May be involved in limb tendon and ligament development.
  • Tissue specificity

    Specifically expressed in skeletal muscle.
  • Involvement in disease

    Defects in SIX1 are the cause of deafness autosomal dominant type 23 (DFNA23) [MIM:605192]. A form of non-syndromic deafness characterized by prelingual, bilateral, symmetric hearing loss with a conductive component present in some but not all patients.
    Defects in SIX1 are the cause of branchiootic syndrome type 3 (BOS3) [MIM:608389]. BOS3 is a syndrome characterized by usually bilateral branchial cleft fistulas or cysts, sensorineural and/or conductive hearing loss, pre-auricular pits, and structural defects of the outer, middle or inner ear. Otic defects include malformed and hypoplastic pinnae, a narrowed external ear canal, bulbous internal auditory canal, stapes fixation, malformed and hypoplastic cochlea. Branchial and otic anomalies are as those seen in individuals with the branchiootorenal syndrome. However, renal anomalies are absent in branchiootic syndrome patients.
    Note=Defects in SIX1 could be a cause of branchiootorenal syndrome (BOR). BOR is an autosomal dominant disorder manifested by various combinations of preauricular pits, branchial fistulae or cysts, lacrimal duct stenosis, hearing loss, structural defects of the outer, middle, or inner ear, and renal dysplasia. Associated defects include asthenic habitus, long narrow facies, constricted palate, deep overbite, and myopia. Hearing loss may be due to mondini type cochlear defect and stapes fixation. Penetrance of BOR syndrome is high, although expressivity can be extremely variable.
  • Sequence similarities

    Belongs to the SIX/Sine oculis homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q15475 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 101128790 Gorilla
    • Entrez Gene: 6495 Human
    • Entrez Gene: 20471 Mouse
    • Omim: 601205 Human
    • SwissProt: A1YER0 Gorilla
    • SwissProt: Q15475 Human
    • SwissProt: Q62231 Mouse
    • Unigene: 54416 Human
    • Unigene: 713114 Human
    • Unigene: 4645 Mouse
    see all
  • Alternative names

    • BOS3 antibody
    • DFNA23 antibody
    • Homeobox protein SIX1 antibody
    • OTTHUMP00000179042 antibody
    • Sine oculis homeobox homolog 1 antibody
    • SIX homeobox 1 antibody
    • SIX1 antibody
    • SIX1_HUMAN antibody
    • TIP39 antibody
    see all

Images

  • Western blot - Anti-SIX1 antibody [CL0185] (ab236761)
    Western blot - Anti-SIX1 antibody [CL0185] (ab236761)
    All lanes : Anti-SIX1 antibody [CL0185] (ab236761) at 1 µg/ml

    Lane 1 : Control siRNA transfected RH-30 cell lysate
    Lane 2 : SIX1 siRNA probe 1 transfected RH-30 cell lysate
    Lane 3 : SIX1 siRNA probe 2 transfected RH-30 cell lysate

    Predicted band size: 32 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [CL0185] (ab236761)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [CL0185] (ab236761)

    Formalin-fixed, paraffin-embedded human striated muscle tissue stained for SIX1 with ab236761 at a 1/200 dilution in immunohistochemical analysis.

    Shows strong nuclear immunoreactivity in the myocytes.

  • Immunocytochemistry/ Immunofluorescence - Anti-SIX1 antibody [CL0185] (ab236761)
    Immunocytochemistry/ Immunofluorescence - Anti-SIX1 antibody [CL0185] (ab236761)

    PFA-fixed, Triton X-100 permeabilized RH-30 cells stained for SIX1 (Green) using ab236761 at 4 μg/ml in ICC/IF.

    Microtublues are stained red.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [CL0185] (ab236761)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [CL0185] (ab236761)

    Formalin-fixed, paraffin-embedded human liver tissue shows absence of immunoreactivity for SIX1 when incubated with ab236761 at a 1/200 dilution (negative control).

  • Western blot - Anti-SIX1 antibody [CL0185] (ab236761)
    Western blot - Anti-SIX1 antibody [CL0185] (ab236761)
    All lanes : Anti-SIX1 antibody [CL0185] (ab236761) at 1 µg/ml

    Lane 1 : RH-30 cell lysate
    Lane 2 : Caco-2 (human colorectal adenocarcinoma cell line) cell lysate

    Predicted band size: 32 kDa

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab236761? Please let us know so that we can cite the reference in this datasheet.

    ab236761 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab236761.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.