Anti-SIX1 antibody [CL0185] (ab236761)
Key features and details
- Mouse monoclonal [CL0185] to SIX1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-SIX1 antibody [CL0185]
See all SIX1 primary antibodies -
Description
Mouse monoclonal [CL0185] to SIX1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Gorilla -
Immunogen
Recombinant fragment corresponding to Human SIX1 aa 130-270.
Sequence:CFKEKSRGVL REWYAHNPYP SPREKRELAE ATGLTTTQVS NWFKNRRQRD RAAEAKEREN TENNNSSSNK QNQLSPLEGG KPLMSSSEEE FSPPQSPDQN SVLLLQGNMG HARSSNYSLP GLTASQPSHG LQTHQHQLQD S
Database link: Q15475 -
Positive control
- ICC/IF: RH-30 cells. WB: RH-30 cell lysate. IHC-P: Human striated muscle tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL0185 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab236761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | 1/200 - 1/500. HIER pH 6 |
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WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa. | |
ICC/IF | Use a concentration of 2 - 10 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
May be involved in limb tendon and ligament development. -
Tissue specificity
Specifically expressed in skeletal muscle. -
Involvement in disease
Defects in SIX1 are the cause of deafness autosomal dominant type 23 (DFNA23) [MIM:605192]. A form of non-syndromic deafness characterized by prelingual, bilateral, symmetric hearing loss with a conductive component present in some but not all patients.
Defects in SIX1 are the cause of branchiootic syndrome type 3 (BOS3) [MIM:608389]. BOS3 is a syndrome characterized by usually bilateral branchial cleft fistulas or cysts, sensorineural and/or conductive hearing loss, pre-auricular pits, and structural defects of the outer, middle or inner ear. Otic defects include malformed and hypoplastic pinnae, a narrowed external ear canal, bulbous internal auditory canal, stapes fixation, malformed and hypoplastic cochlea. Branchial and otic anomalies are as those seen in individuals with the branchiootorenal syndrome. However, renal anomalies are absent in branchiootic syndrome patients.
Note=Defects in SIX1 could be a cause of branchiootorenal syndrome (BOR). BOR is an autosomal dominant disorder manifested by various combinations of preauricular pits, branchial fistulae or cysts, lacrimal duct stenosis, hearing loss, structural defects of the outer, middle, or inner ear, and renal dysplasia. Associated defects include asthenic habitus, long narrow facies, constricted palate, deep overbite, and myopia. Hearing loss may be due to mondini type cochlear defect and stapes fixation. Penetrance of BOR syndrome is high, although expressivity can be extremely variable. -
Sequence similarities
Belongs to the SIX/Sine oculis homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 101128790 Gorilla
- Entrez Gene: 6495 Human
- Entrez Gene: 20471 Mouse
- Omim: 601205 Human
- SwissProt: A1YER0 Gorilla
- SwissProt: Q15475 Human
- SwissProt: Q62231 Mouse
- Unigene: 54416 Human
see all -
Alternative names
- BOS3 antibody
- DFNA23 antibody
- Homeobox protein SIX1 antibody
see all
Images
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All lanes : Anti-SIX1 antibody [CL0185] (ab236761) at 1 µg/ml
Lane 1 : Control siRNA transfected RH-30 cell lysate
Lane 2 : SIX1 siRNA probe 1 transfected RH-30 cell lysate
Lane 3 : SIX1 siRNA probe 2 transfected RH-30 cell lysate
Predicted band size: 32 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [CL0185] (ab236761)
Formalin-fixed, paraffin-embedded human striated muscle tissue stained for SIX1 with ab236761 at a 1/200 dilution in immunohistochemical analysis.
Shows strong nuclear immunoreactivity in the myocytes.
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PFA-fixed, Triton X-100 permeabilized RH-30 cells stained for SIX1 (Green) using ab236761 at 4 μg/ml in ICC/IF.
Microtublues are stained red.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIX1 antibody [CL0185] (ab236761)
Formalin-fixed, paraffin-embedded human liver tissue shows absence of immunoreactivity for SIX1 when incubated with ab236761 at a 1/200 dilution (negative control).
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All lanes : Anti-SIX1 antibody [CL0185] (ab236761) at 1 µg/ml
Lane 1 : RH-30 cell lysate
Lane 2 : Caco-2 (human colorectal adenocarcinoma cell line) cell lysate
Predicted band size: 32 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab236761 has not yet been referenced specifically in any publications.