Recombinant Anti-SIX2 antibody [EPR4824] (ab111827)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4824] to SIX2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-SIX2 antibody [EPR4824]
See all SIX2 primary antibodies -
Description
Rabbit monoclonal [EPR4824] to SIX2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Skeletal Muscle (Rat) Nuclear Lysate - normal tissue (ab29375) can be used as a positive control in WB. Human, Mouse and Rat skeletal muscle lysates; L6 lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4824 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 32 kDa. |
Target
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Function
May be involved in limb tendon and ligament development. -
Tissue specificity
Strongly expressed in skeletal muscle. -
Sequence similarities
Belongs to the SIX/Sine oculis homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10736 Human
- Entrez Gene: 20472 Mouse
- Entrez Gene: 366542 Rat
- Omim: 604994 Human
- SwissProt: Q9NPC8 Human
- SwissProt: Q62232 Mouse
- Unigene: 101937 Human
- Unigene: 5039 Mouse
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Alternative names
- Homeobox protein SIX2 antibody
- OTTHUMP00000201649 antibody
- Sine oculis homeobox (Drosophila) homolog 2 antibody
see all
Images
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All lanes : Anti-SIX2 antibody [EPR4824] (ab111827) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Mouse skeletal muscle lysate
Lane 3 : Rat skeletal muscle lysate
Lane 4 : L6 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 32 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab111827 has been referenced in 2 publications.
- Creed TM et al. The PAX-SIX-EYA-DACH network modulates GATA-FOG function in fly hematopoiesis and human erythropoiesis. Development 147:N/A (2020). PubMed: 31806659
- Jia Q et al. Circular RNA 0007255 regulates the progression of breast cancer through miR-335-5p/SIX2 axis. Thorac Cancer 11:619-630 (2020). PubMed: 31962380