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Our Abpromise guarantee covers the use of ab176713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 83 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
SIX4 was immunoprecipitated from 1mg HeLa whole cell lysate using ab176713 at 6 μg/mg lysate (Lane 2), a rabbit anti-SIX4 antibody recognizing an upstream epitope (Lane 1) or control IgG (Lane 3). 20% of the Immunoprecipitate was loaded per lane and then immunobotted using ab176713 at 0.4 μg/ml.
Detection: Chemiluminescence with exposure time of 3 seconds.
ab176713 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"