Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization.
Belongs to the SKA3 family.
Cytoplasm > cytoskeleton > spindle. Chromosome > centromere > kinetochore. Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner.
Detection of Human SKA3 by Western Blot of Immunoprecipitates. 1 mg for IP; 20% of IP Jurkat whole cell lysate loaded. ab186003 used for IP at 6µg/ml. For blotting immunoprecipitated SKA3, ab186003 was used at 1 µg/ml.
Western blot - Anti-SKA3 antibody - C-terminal (ab186003)
All lanes : Anti-SKA3 antibody - C-terminal (ab186003) at 0.1 µg/ml
Lane 1 : HeLa lysate Lane 2 : 293T lysate Lane 3 : Jurkat lysate