Key features and details
- Rabbit polyclonal to SKA3 - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SKA3 antibody - C-terminal
See all SKA3 primary antibodies
DescriptionRabbit polyclonal to SKA3 - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
- HeLa, 293T or jurkat lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab186003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 43 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionComponent of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization.
Sequence similaritiesBelongs to the SKA3 family.
Cellular localizationCytoplasm > cytoskeleton > spindle. Chromosome > centromere > kinetochore. Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner.
- Information by UniProt
- C13orf3 antibody
- RAMA1 antibody
- SKA3 antibody
Detection of Human SKA3 by Western Blot of Immunoprecipitates. 1 mg for IP; 20% of IP Jurkat whole cell lysate loaded. ab186003 used for IP at 6µg/ml. For blotting immunoprecipitated SKA3, ab186003 was used at 1 µg/ml.
All lanes : Anti-SKA3 antibody - C-terminal (ab186003) at 0.1 µg/ml
Lane 1 : HeLa lysate
Lane 2 : 293T lysate
Lane 3 : Jurkat lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 43 kDa
Exposure time: 30 seconds
ab186003 has not yet been referenced specifically in any publications.