Product nameAnti-SKAP antibody
DescriptionRabbit polyclonal to SKAP
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- Human kidney tissue; RT-4 and U251-MG lysate
Previously labelled as TRAF4AF1.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|WB||1/1000 - 1/2500.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
- Information by UniProt
- KNSTRN antibody
- C15orf23 antibody
- chromosome 15 open reading frame 23 antibody
All lanes : Anti-SKAP antibody (ab122769) at 1/250 dilution
Lane 1 : RT-4 lysate
Lane 2 : U251-MG lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm and cytoskeleton (microtubules). Recommended concentration of ab122769 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122769, at 1/50 dilution, staining SKAP in paraffin-embedded Human kidney tissue by Immunohistochemistry.
This product has been referenced in:
- Lu S et al. Small kinetochore associated protein (SKAP) promotes UV-induced cell apoptosis through negatively regulating pre-mRNA processing factor 19 (Prp19). PLoS One 9:e92712 (2014). WB ; Human . Read more (PubMed: 24718257) »
- Lee CS et al. Recurrent point mutations in the kinetochore gene KNSTRN in cutaneous squamous cell carcinoma. Nat Genet 46:1060-2 (2014). Read more (PubMed: 25194279) »