Product nameAnti-SKAP55 antibody
See all SKAP55 primary antibodies
DescriptionRabbit polyclonal to SKAP55
SpecificityDetects Human SKAP55.
Tested applicationsSuitable for: ICC, IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rat
- Human Jurkat cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab19377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml. Detects an ~ 60 kDa protein from Human Jurkat cells.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPositively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells.
Tissue specificityHighly expressed in thymocytes and peripheral blood lymphocytes. Also expressed in spleen cells and testis. Present in T-cells (at protein level).
Sequence similaritiesBelongs to the SKAP family.
Contains 1 PH domain.
Contains 1 SH3 domain.
DomainThe SH3 domain interacts with FYB.
modificationsPhosphorylated on tyrosines. Phosphorylation by FYN on Tyr-271 is required for GRB2 interaction. Phosphorylation by FYN on Tyr-295 abolishes interaction with FYB. Tyr-232 is dephosphorylated by PTPRC.
Cellular localizationCytoplasm. Nucleus. Cell membrane. Upon T-cell stimulation, translocates to lipid rafts at the cell membrane.
- Information by UniProt
- pp55 antibody
- SCAP 1 antibody
- SCAP1 antibody
All lanes : Anti-SKAP55 antibody (ab19377) at 1/500 dilution
Lane 1 : Human T cell lysate
Lane 2 : Human platelet lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG conjugated to Odyssey IR-Dye 800 at 1/50000 dilution
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa (possible non-specific binding)
Exposure time: 3 minutes
Detection: Licor Odyssey system
Blocking: 3% BSA
ab19377 has not yet been referenced specifically in any publications.