Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11359] to SKAP55
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-SKAP55 antibody [EPR11359]
See all SKAP55 primary antibodies
DescriptionRabbit monoclonal [EPR11359] to SKAP55
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human SKAP55 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: Q86WV1
- Molt4 cells, Hut 78 cell line lysate, Human thymus tissue lysate,
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab171947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 41 kDa.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPositively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells.
Tissue specificityHighly expressed in thymocytes and peripheral blood lymphocytes. Also expressed in spleen cells and testis. Present in T-cells (at protein level).
Sequence similaritiesBelongs to the SKAP family.
Contains 1 PH domain.
Contains 1 SH3 domain.
DomainThe SH3 domain interacts with FYB.
modificationsPhosphorylated on tyrosines. Phosphorylation by FYN on Tyr-271 is required for GRB2 interaction. Phosphorylation by FYN on Tyr-295 abolishes interaction with FYB. Tyr-232 is dephosphorylated by PTPRC.
Cellular localizationCytoplasm. Nucleus. Cell membrane. Upon T-cell stimulation, translocates to lipid rafts at the cell membrane.
- Information by UniProt
- pp55 antibody
- SCAP 1 antibody
- SCAP1 antibody
All lanes : Anti-SKAP55 antibody [EPR11359] (ab171947) at 1/10000 dilution
Lane 1 : Molt4 cell line lysate
Lane 2 : HuT 78 cell line lysate
Lane 3 : Human thymus tissue lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 41 kDa
Flow cytometric analysis of permeabilized Molt4 labeling SKAP55 with ab171947 at 1/100 (red) or a rabbit IgG (negative) (green).
ab171947 has not yet been referenced specifically in any publications.