Product nameAnti-SKAP55 antibody [EPR11360]
See all SKAP55 primary antibodies
DescriptionRabbit monoclonal [EPR11360] to SKAP55
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SKAP55 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q86WV1
- Human thymus, Jurkat, HuT-78 and MOLT4 lysates, Jurkat cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab170882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 41 kDa.|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPositively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells.
Tissue specificityHighly expressed in thymocytes and peripheral blood lymphocytes. Also expressed in spleen cells and testis. Present in T-cells (at protein level).
Sequence similaritiesBelongs to the SKAP family.
Contains 1 PH domain.
Contains 1 SH3 domain.
DomainThe SH3 domain interacts with FYB.
modificationsPhosphorylated on tyrosines. Phosphorylation by FYN on Tyr-271 is required for GRB2 interaction. Phosphorylation by FYN on Tyr-295 abolishes interaction with FYB. Tyr-232 is dephosphorylated by PTPRC.
Cellular localizationCytoplasm. Nucleus. Cell membrane. Upon T-cell stimulation, translocates to lipid rafts at the cell membrane.
- Information by UniProt
- pp55 antibody
- SCAP 1 antibody
- SCAP1 antibody
All lanes : Anti-SKAP55 antibody [EPR11360] (ab170882) at 1/1000 dilution
Lane 1 : Human thymus lysate
Lane 2 : Jurkat lysate
Lane 3 : HuT-78 lysate
Lane 4 : MOLT4 lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 41 kDa
Immunofluorescent staining of Jurkat cells labeling SKAP55 using ab170882 at a 1/100 dilution (green) and DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized Jurkat cells labeling SKAP55 using ab170882 at a 1/10 dilution (red) or a rabbit IgG (green).
ab170882 has not yet been referenced specifically in any publications.