Recombinant Anti-SKAP55 antibody [EPR11360] (ab170882)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11360] to SKAP55
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SKAP55 antibody [EPR11360]
See all SKAP55 primary antibodies -
Description
Rabbit monoclonal [EPR11360] to SKAP55 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human thymus, Jurkat, HuT-78 and MOLT4 lysates, Jurkat cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11360 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
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ICC/IF |
1/100 - 1/250.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells. -
Tissue specificity
Highly expressed in thymocytes and peripheral blood lymphocytes. Also expressed in spleen cells and testis. Present in T-cells (at protein level). -
Sequence similarities
Belongs to the SKAP family.
Contains 1 PH domain.
Contains 1 SH3 domain. -
Domain
The SH3 domain interacts with FYB. -
Post-translational
modificationsPhosphorylated on tyrosines. Phosphorylation by FYN on Tyr-271 is required for GRB2 interaction. Phosphorylation by FYN on Tyr-295 abolishes interaction with FYB. Tyr-232 is dephosphorylated by PTPRC. -
Cellular localization
Cytoplasm. Nucleus. Cell membrane. Upon T-cell stimulation, translocates to lipid rafts at the cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 8631 Human
- Entrez Gene: 78473 Mouse
- Entrez Gene: 286975 Rat
- Omim: 604969 Human
- SwissProt: Q86WV1 Human
- SwissProt: Q3UUV5 Mouse
- SwissProt: Q4V7G1 Rat
- Unigene: 316931 Human
see all -
Alternative names
- pp55 antibody
- SCAP 1 antibody
- SCAP1 antibody
see all
Images
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All lanes : Anti-SKAP55 antibody [EPR11360] (ab170882) at 1/1000 dilution
Lane 1 : Human thymus lysate
Lane 2 : Jurkat lysate
Lane 3 : HuT-78 lysate
Lane 4 : MOLT4 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 41 kDa -
Immunofluorescent staining of Jurkat cells labeling SKAP55 using ab170882 at a 1/100 dilution (green) and DAPI nuclear staining (blue).
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Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling SKAP55using ab170882 at a 1/10 dilution (red) or a rabbit IgG (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab170882 has not yet been referenced specifically in any publications.