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Rabbit polyclonal to SKI
Predicted to work with:
Rat, Horse, Chicken, Dog, Xenopus laevis
Synthetic peptide conjugated to KLH derived from within residues 600 - 700 of Human c-Ski.
(Peptide available as
This antibody gave a positive signal in Hela Whole Cell Lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 80 kDa).
May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling.
Belongs to the SKI family.
Information by UniProt
Avian sarcoma viral (v ski) oncogene homolog antibody
C oncogene antibody
C ski antibody
Western blot - Anti-SKI antibody (ab19864)
Anti-SKI antibody (ab19864) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size:
Observed band size:
85 kDa (
why is the actual band size different from the predicted?
Additional bands at:
49 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Anti-SKI antibody (ab19864)
ICC/IF image of ab19864 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19864, 5µg/ml) overnight at +4°C. The secondary antibody (green) was
Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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