Product nameAnti-SKI antibody
See all SKI primary antibodies
DescriptionRabbit polyclonal to SKI
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Chicken, Dog, Xenopus laevis
- This antibody gave a positive signal in Hela Whole Cell Lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab19864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 80 kDa).|
FunctionMay play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling.
Sequence similaritiesBelongs to the SKI family.
- Information by UniProt
- Avian sarcoma viral (v ski) oncogene homolog antibody
- C oncogene antibody
- C ski antibody
Anti-SKI antibody (ab19864) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Additional bands at: 49 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 15 minutes
ICC/IF image of ab19864 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19864, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Hatley ME et al. Modulation of K-Ras-Dependent Lung Tumorigenesis by MicroRNA-21. Cancer Cell 18:282-93 (2010). WB ; Mouse . Read more (PubMed: 20832755) »
- O TM et al. Differential expression of SKI oncogene protein in hemangiomas. Otolaryngol Head Neck Surg 141:213-8 (2009). Read more (PubMed: 19643254) »