Overview

  • Product name

    Anti-SKI antibody - C-terminal
    See all SKI primary antibodies
  • Description

    Rabbit polyclonal to SKI - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human SKI aa 678-728 (C terminal). The exact sequence is proprietary. NP_003027.1
    Database link: P12755

  • Positive control

    • IP: HEK-293T whole cell lysate. WB: HEK-293T, Jurkat and HeLa whole cell lysate (ab150035).

Properties

Applications

Our Abpromise guarantee covers the use of ab226208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 80 kDa.

Target

  • Function

    May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling.
  • Sequence similarities

    Belongs to the SKI family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Avian sarcoma viral (v ski) oncogene homolog antibody
    • C oncogene antibody
    • C ski antibody
    • Proto-oncogene c-Ski antibody
    • SKI antibody
    • Ski oncogene antibody
    • Ski oncoprotein antibody
    • SKI_HUMAN antibody
    • SKV antibody
    • v ski avian sarcoma viral oncogene homolog antibody
    • v ski sarcoma viral oncogene homolog antibody
    see all

Images

  • All lanes : Anti-SKI antibody - C-terminal (ab226208) at 0.04 µg/ml

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
    Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 80 kDa


    Exposure time: 3 minutes
  • SKI was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226208 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226208 at 1 µg/ml.

    Lane 1: ab226208 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 10 seconds.

References

ab226208 has not yet been referenced specifically in any publications.

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