Product nameAnti-SKI antibody - C-terminal
See all SKI primary antibodies
DescriptionRabbit polyclonal to SKI - C-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human SKI aa 678-728 (C terminal). The exact sequence is proprietary. NP_003027.1
Database link: P12755
- IP: HEK-293T whole cell lysate. WB: HEK-293T, Jurkat and HeLa whole cell lysate (ab150035).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 80 kDa.|
FunctionMay play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling.
Sequence similaritiesBelongs to the SKI family.
- Information by UniProt
- Avian sarcoma viral (v ski) oncogene homolog antibody
- C oncogene antibody
- C ski antibody
All lanes : Anti-SKI antibody - C-terminal (ab226208) at 0.04 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 80 kDa
Exposure time: 3 minutes
SKI was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226208 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226208 at 1 µg/ml.
Lane 1: ab226208 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
ab226208 has not yet been referenced specifically in any publications.