Overview

  • Product name

  • Description

    Rabbit polyclonal to Ski8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Chicken, Cow, Human, Xenopus laevis, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Ski8 (internal sequence). The exact sequence is proprietary.
    Database link: Q9GZS3

  • Positive control

    • IHC-P: Rat brain and hindbrain tissue. Mouse forebrain and pancreas tissue.
  • General notes

    Previously labelled as WDR61. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.

Target

  • Function

    Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of MLL1; it promotes leukemogenesis though association with MLL-rearranged oncoproteins, such as MLL-MLLT3/AF9 and MLL-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3), dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C.
  • Sequence similarities

    Contains 7 WD repeats.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Meiotic recombination REC14 protein homolog antibody
    • REC14 antibody
    • Recombination protein REC14 antibody
    • Ski8 antibody
    • SKI8 homolog antibody
    • WD repeat containing protein 61 antibody
    • WD repeat domain 61 antibody
    • WD repeat-containing protein 61 antibody
    • WDR 61 antibody
    • wdr61 antibody
    • WDR61_HUMAN antibody
    see all

Images

  • Paraffin-embedded rat brain tissue stained for Ski8 with ab228851 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse pancreas tissue stained for Ski8 with ab228851 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse forebrain tissue stained for Ski8 with ab228851 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat hindbrain tissue stained for Ski8 with ab228851 at 1/500 dilution in immunohistochemical analysis.

References

ab228851 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228851.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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