Key features and details
- Rabbit polyclonal to Ski8
- Suitable for: WB
- Reacts with: Human, Drosophila melanogaster
- Isotype: IgG
Product nameAnti-Ski8 antibody
See all Ski8 primary antibodies
DescriptionRabbit polyclonal to Ski8
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human, Drosophila melanogaster
Recombinant fragment corresponding to Human Ski8.
Database link: Q9GZS3
- WB: HeLa nuclear extract; Drosophila adult whole cell extracts; Purified human PAF complex.
Previously labelled as WDR61.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.05% Sodium azide, 0.05% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab231295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 34 kDa.|
FunctionComponent of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of MLL1; it promotes leukemogenesis though association with MLL-rearranged oncoproteins, such as MLL-MLLT3/AF9 and MLL-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3), dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C.
Sequence similaritiesContains 7 WD repeats.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- Meiotic recombination REC14 protein homolog antibody
- REC14 antibody
- Recombination protein REC14 antibody
Anti-Ski8 antibody (ab231295) at 1/1000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear extract at 20 µg
Predicted band size: 34 kDa
All lanes : Anti-Ski8 antibody (ab231295) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear extract
Lane 2 : Drosophila adult whole cell extracts
Lane 3 : Purified human PAF complex
Predicted band size: 34 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231295 has not yet been referenced specifically in any publications.