Overview

  • Product name

  • Description

    Rabbit polyclonal to Ski8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human, Drosophila melanogaster
  • Immunogen

    Recombinant fragment corresponding to Human Ski8.
    Database link: Q9GZS3

  • Positive control

    • WB: HeLa nuclear extract; Drosophila adult whole cell extracts; Purified human PAF complex.
  • General notes

    Previously labelled as WDR61. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservatives: 0.05% Sodium azide, 0.05% Proclin
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab231295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 34 kDa.

Target

  • Function

    Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of MLL1; it promotes leukemogenesis though association with MLL-rearranged oncoproteins, such as MLL-MLLT3/AF9 and MLL-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3), dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C.
  • Sequence similarities

    Contains 7 WD repeats.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Meiotic recombination REC14 protein homolog antibody
    • REC14 antibody
    • Recombination protein REC14 antibody
    • Ski8 antibody
    • SKI8 homolog antibody
    • WD repeat containing protein 61 antibody
    • WD repeat domain 61 antibody
    • WD repeat-containing protein 61 antibody
    • WDR 61 antibody
    • wdr61 antibody
    • WDR61_HUMAN antibody
    see all

Images

  • Anti-Ski8 antibody (ab231295) at 1/1000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear extract at 20 µg

    Predicted band size: 34 kDa

  • All lanes : Anti-Ski8 antibody (ab231295) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear extract
    Lane 2 : Drosophila adult whole cell extracts
    Lane 3 : Purified human PAF complex

    Predicted band size: 34 kDa

References

ab231295 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab231295.
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