Key features and details
- Mouse polyclonal to SKIP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab67715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 51 kDa.|
FunctionInositol 5-phosphatase which acts on inositol 1,4,5-trisphosphate, inositol 1,3,4,5-tetrakisphosphate, phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-triphosphate. Has 6-fold higher affinity for phosphatidylinositol 4,5-bisphosphate than for inositol 1,4,5-trisphosphate. May negatively regulate assembly of the actin cytoskeleton.
Tissue specificityUbiquitously expressed with highest levels in skeletal muscle, heart and kidney.
Sequence similaritiesBelongs to the inositol-1,4,5-trisphosphate 5-phosphatase type II family.
Cellular localizationEndoplasmic reticulum. Following stimulation with EGF, translocates to membrane ruffles.
- Information by UniProt
- 43 kDa form skeletal muscle and kidney enriched inositol phosphatase antibody
- Inositol polyphosphate 5-phosphatase K antibody
- INP5K_HUMAN antibody
All lanes : Anti-SKIP antibody (ab67715) at 1/500 dilution
Lane 1 : SKIP transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
ab67715 has been referenced in 1 publication.
- Wu MY et al. SNW1 is a critical regulator of spatial BMP activity, neural plate border formation, and neural crest specification in vertebrate embryos. PLoS Biol 9:e1000593 (2011). WB . PubMed: 21358802