Product nameAnti-Skp1 antibody
See all Skp1 primary antibodies
DescriptionRabbit polyclonal to Skp1
SpecificityThis antibody detects a 19kD band in Hela and HEK293 cells. The band can be blocked with the immunising peptide. The antibody shows extensive staining (eg. of lymphoid and epithelial cells), with staining of nuclei and some staining of the cytoplasm, when tested on human tonsil in IHC.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Guinea pig, Xenopus laevis, Zebrafish
Synthetic peptide derived from residues 50 - 150 of Human Skp1.
- Recombinant Human Skp1 protein (ab117211) can be used as a positive control in WB. This antibody gave a positive signal in HeLa nuclear lysate as well as the following whole cell lysates: HeLa; HEK293. IF/ICC: MCF7 cell line
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab10546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 19 kDa (predicted molecular weight: 18.5 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionEssential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the SKP1 family.
- Information by UniProt
- Cyclin A/CDK2 associated p19 antibody
- Cyclin A/CDK2 associated protein p19 antibody
- Cyclin-A/CDK2-associated protein p19 antibody
Western blot using ab10546 at 1/500.
Lane 1: Hela nuclear
Lane 2: Hela
Lane 3: HEK293
Lane 4: Hela nuclear + ab10546 blocking peptide (1µg/ml)
Lane 5: Hela + ab10546 blocking peptide (1µg/ml)
Lane 6: HEK293 + ab10546 blocking peptide (1µg/ml)
Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
Exposure time: 2 mins.
ICC/IF image of ab10546 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10546, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab10546 was used in immunohistochemistry with paraffin embedded sections of human tonsil, using DAB as a chromogen (brown). Counterstaining of nuclei was performed with haemotoxylin (blue).
Strong staining of epithelial cell nuclei is seen (and some staining of the cytoplasm) in this image.
This product has been referenced in:
- Bruckert WM et al. Rapid nutritional remodeling of the host cell upon attachment of Legionella pneumophila. Infect Immun 82:72-82 (2014). Read more (PubMed: 24126522) »
- Viñas-Castells R et al. Nuclear ubiquitination by FBXL5 modulates Snail1 DNA binding and stability. Nucleic Acids Res N/A:N/A (2013). WB . Read more (PubMed: 24157836) »