Anti-Skp1 antibody (ab228637)
Key features and details
- Rabbit polyclonal to Skp1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Skp1 antibody
See all Skp1 primary antibodies -
Description
Rabbit polyclonal to Skp1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Chicken, Guinea pig, Cow, Dog, Xenopus laevis, Zebrafish, Rhesus monkey -
Immunogen
Recombinant fragment within Human Skp1 (internal sequence). The exact sequence is proprietary.
Database link: P63208 -
Positive control
- WB: NIH/3T3, A549, NCI-H1299, HCT 116 and MCF7 whole cell lysates. IHC-P: Mouse middle brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 19 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 19 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Function
Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the SKP1 family. - Information by UniProt
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Database links
- Entrez Gene: 416319 Chicken
- Entrez Gene: 615427 Cow
- Entrez Gene: 474682 Dog
- Entrez Gene: 100135616 Guinea pig
- Entrez Gene: 6500 Human
- Entrez Gene: 21402 Mouse
- Entrez Gene: 287280 Rat
- Entrez Gene: 100423634 Rhesus monkey
see all -
Alternative names
- Cyclin A/CDK2 associated p19 antibody
- Cyclin A/CDK2 associated protein p19 antibody
- Cyclin-A/CDK2-associated protein p19 antibody
see all
Images
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Paraffin-embedded mouse middle brain tissue stained for Skp1 with ab228637 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-Skp1 antibody (ab228637) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 19 kDa12% SDS-PAGE
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Anti-Skp1 antibody (ab228637) at 1/3000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 19 kDa12% SDS-PAGE
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228637 has not yet been referenced specifically in any publications.