Overview

  • Product name

  • Description

    Rabbit polyclonal to Skp1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Chicken, Guinea pig, Cow, Dog, Xenopus laevis, Zebrafish, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Skp1 (internal sequence). The exact sequence is proprietary.
    Database link: P63208

  • Positive control

    • WB: NIH/3T3, A549, NCI-H1299, HCT 116 and MCF7 whole cell lysates. IHC-P: Mouse middle brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab228637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 19 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the SKP1 family.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cyclin A/CDK2 associated p19 antibody
    • Cyclin A/CDK2 associated protein p19 antibody
    • Cyclin-A/CDK2-associated protein p19 antibody
    • EMC19 antibody
    • MGC34403 antibody
    • OCP 2 antibody
    • OCP II antibody
    • OCP II protein antibody
    • OCP-2 antibody
    • OCP-II antibody
    • OCP2 antibody
    • OCPII antibody
    • Organ of Corti protein 2 antibody
    • Organ of Corti protein II antibody
    • OTTHUMP00000159379 antibody
    • OTTHUMP00000159380 antibody
    • OTTHUMP00000228275 antibody
    • OTTHUMP00000228276 antibody
    • OTTHUMP00000228278 antibody
    • OTTHUMP00000228279 antibody
    • OTTHUMP00000228281 antibody
    • p19A antibody
    • p19skp1 antibody
    • RNA polymerase II elongation factor like protein antibody
    • RNA polymerase II elongation factor like protein OCP2 antibody
    • RNA polymerase II elongation factor-like protein antibody
    • S phase kinase associated protein 1 antibody
    • S phase kinase associated protein 1A antibody
    • S phase kinase associated protein 1A p19A antibody
    • S-phase kinase-associated protein 1 antibody
    • SIII antibody
    • Skp 1 antibody
    • SKP 1A antibody
    • skp1 antibody
    • SKP1_HUMAN antibody
    • SKP1A antibody
    • TCEB1L antibody
    • Transcription Elongation Factor B 1 like antibody
    • Transcription elongation factor B antibody
    • Transcription elongation factor B SIII polypeptide 1 like antibody
    see all

Images

  • Paraffin-embedded mouse middle brain tissue stained for Skp1 with ab228637 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-Skp1 antibody (ab228637) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
    Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
    Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 19 kDa



    12% SDS-PAGE

  • Anti-Skp1 antibody (ab228637) at 1/3000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 19 kDa



    12% SDS-PAGE

References

ab228637 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228637.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up