• Product name

    Anti-Skp1 antibody [EPR3303]
    See all Skp1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3303] to Skp1
  • Host species

  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Pmore details
    Unsuitable for: ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Skp1. The exact sequence is proprietary.

  • Positive control

    • WB: lysates from 293, HeLa, PC12 or 3T3 cells; IHC-P: Human brain tissue; FC: permeabilized HeLa cells.
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab80586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: 1/50.
    IHC-P: 1/100 - 1/250. Antigen retrieval is recommended.
    WB: 1/1000 - 1/5000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).

    Is unsuitable for ICC or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Belongs to the SKP1 family.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cyclin A/CDK2 associated p19 antibody
      • Cyclin A/CDK2 associated protein p19 antibody
      • Cyclin-A/CDK2-associated protein p19 antibody
      • EMC19 antibody
      • MGC34403 antibody
      • OCP 2 antibody
      • OCP II antibody
      • OCP II protein antibody
      • OCP-2 antibody
      • OCP-II antibody
      • OCP2 antibody
      • OCPII antibody
      • Organ of Corti protein 2 antibody
      • Organ of Corti protein II antibody
      • OTTHUMP00000159379 antibody
      • OTTHUMP00000159380 antibody
      • OTTHUMP00000228275 antibody
      • OTTHUMP00000228276 antibody
      • OTTHUMP00000228278 antibody
      • OTTHUMP00000228279 antibody
      • OTTHUMP00000228281 antibody
      • p19A antibody
      • p19skp1 antibody
      • RNA polymerase II elongation factor like protein antibody
      • RNA polymerase II elongation factor like protein OCP2 antibody
      • RNA polymerase II elongation factor-like protein antibody
      • S phase kinase associated protein 1 antibody
      • S phase kinase associated protein 1A antibody
      • S phase kinase associated protein 1A p19A antibody
      • S-phase kinase-associated protein 1 antibody
      • SIII antibody
      • Skp 1 antibody
      • SKP 1A antibody
      • skp1 antibody
      • SKP1_HUMAN antibody
      • SKP1A antibody
      • TCEB1L antibody
      • Transcription Elongation Factor B 1 like antibody
      • Transcription elongation factor B antibody
      • Transcription elongation factor B SIII polypeptide 1 like antibody
      see all


    • All lanes : Anti-Skp1 antibody [EPR3303] (ab80586) at 1/5000 dilution

      Lane 1 : 293 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : PC12 cell lysate
      Lane 4 : 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 19 kDa
      Observed band size: 19 kDa

    • Immunohistochemistry analysis of paraffin-embedded Human brain tissue with 1/100 ab80586.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • ab80586 at 1/50 dilution (red) staining Skp1 in permeabilized HeLa cells by Flow cytometry. Rabbit IgG (negative) is shown in green.


    This product has been referenced in:

    • Wang D  et al. Inhibition of S-phase kinase-associated protein 2 (Skp2) reprograms and converts diabetogenic T cells to Foxp3+ regulatory T cells. Proc Natl Acad Sci U S A 109:9493-8 (2012). WB ; Mouse . Read more (PubMed: 22645357) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Immunocytochemistry/ Immunofluorescence
    Human Cell (HeLa)
    Yes - 0.5% Triton X100 in PBS

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Jul 10 2014

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