Product nameAnti-Skp1 antibody [EPR3303]
See all Skp1 primary antibodies
DescriptionRabbit monoclonal [EPR3303] to Skp1
Tested applicationsSuitable for: WB, Flow Cyt, IHC-Pmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Skp1. The exact sequence is proprietary.
- WB: lysates from 293, HeLa, PC12 or 3T3 cells; IHC-P: Human brain tissue; FC: permeabilized HeLa cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA, 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab80586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/250. Antigen retrieval is recommended.
WB: 1/1000 - 1/5000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
Is unsuitable for ICC or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionEssential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the SKP1 family.
- Information by UniProt
- Cyclin A/CDK2 associated p19 antibody
- Cyclin A/CDK2 associated protein p19 antibody
- Cyclin-A/CDK2-associated protein p19 antibody
All lanes : Anti-Skp1 antibody [EPR3303] (ab80586) at 1/5000 dilution
Lane 1 : 293 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa
Immunohistochemistry analysis of paraffin-embedded Human brain tissue with 1/100 ab80586.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
ab80586 at 1/50 dilution (red) staining Skp1 in permeabilized HeLa cells by Flow cytometry. Rabbit IgG (negative) is shown in green.
This product has been referenced in:
- Wang D et al. Inhibition of S-phase kinase-associated protein 2 (Skp2) reprograms and converts diabetogenic T cells to Foxp3+ regulatory T cells. Proc Natl Acad Sci U S A 109:9493-8 (2012). WB ; Mouse . Read more (PubMed: 22645357) »