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Dear Abcam, I recently purchased your rabbit anti Skp2 antibody (ab19877). I have now tested this antibody on western blots containing rat, human and mouse cell lysates and compared it to [another company] anti Skp2 antibody using the sample samples run at the same time. I would like to complain that your antibody does not recognise Skp2 in rat, mouse or human cell lysates. As you can see from the scans of the Abcam and [another company] blots, Skp2 should be absent in serum deprived cells, strongly induced in FCS stimulated cells and present in asynchronously proliferating cells. This is clearly evident in the strong Skp2 band int he [another company] blot but not at all on the blot using the Abcam antibody. The band in the Abcam blot running at slightly high than the 45k marker is a non-specific band. I would therefore like to request a refund on this antibody as it clearly is not suitable for use in detecting Skp2 on western blots with human or mouse cell lysates. My order number when I originally placed this order was BHI-12057 and the invoice number was 1029721. Thank you for your help,
Asked on Jul 10 2006
I'm very sorry to hear you are experiencing problems with ab19877. I take your comments very seriously and would appreciate if you could please clarify the conditions you have used the antibody in. It is clear that your samples express high levels of SKP2, therefore there is no doubt that the problem is due to either the quality of the antibody or the conditions it has been used. To eliminate the second possibility as a reason can you please clarify: -what blocking agent was used -what antibody dilution buffer was used -how long the antibody was incubated for -what dilutions were tested Please be assured that should the problem be due to the quality of the product I can certainly arrange for a credit note to be issued to pay for the order 156940 (BHI-12057). I apologise for the delay in resolving this matter and thank you in advance for providing the above information needed,
Answered on Jul 12 2006