Anti-SKP2 antibody (ab216687)
Key features and details
- Rabbit polyclonal to SKP2
- Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SKP2 antibody
See all SKP2 primary antibodies -
Description
Rabbit polyclonal to SKP2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human SKP2 aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q13309 -
Positive control
- WB: 293T cell lysate. IHC-P: Human thyroid carcinoma tissue. ICC/IF: MCF-7 cells. Flow Cyt (Intra): HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/500.
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Flow Cyt (Intra) |
1/100.
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WB |
1/500.
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Notes |
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IHC-P
1/100 - 1/500. |
ICC/IF
1/500. |
Flow Cyt (Intra)
1/100. |
WB
1/500. |
Target
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Function
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1A, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 F-box domain.
Contains 9 LRR (leucine-rich) repeats. - Information by UniProt
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Database links
- Entrez Gene: 6502 Human
- Entrez Gene: 27401 Mouse
- Entrez Gene: 294790 Rat
- Omim: 601436 Human
- SwissProt: Q13309 Human
- SwissProt: Q9Z0Z3 Mouse
- Unigene: 23348 Human
- Unigene: 35584 Mouse
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Alternative names
- CDK2/Cyclin A associated protein p45 antibody
- Cyclin A/CDK2 associated protein p45 antibody
- Cyclin-A/CDK2-associated protein p45 antibody
see all
Images
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Anti-SKP2 antibody (ab216687) at 1/500 dilution + 293T lysates
Secondary
Conjugated secondary antibody at 1/10000 dilution -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thyroid carcinoma tissue labeling SKP2 with ab216687 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Immunofluorescent analysis of MCF-7 cells labeling SKP2 with ab216687 at 1/500 dilution in PBS for two hours at 37°C, followed by Goat anti-rabbit IgG (H+L), Cy3 conjugated secondary antibody. The nucleus was stained with DAPI.
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Intracellular flow cytometric analysis of HeLa cells labeling SKP2 with ab 216687 at 1/100 dilution for 30 minutes followed by a PE conjugated secondary antibody (green), compared to unstained cells (blue), secondary only (light blue), and an isotype control (orange).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab216687 has not yet been referenced specifically in any publications.