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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin E3 Enzymes F Box E3 Ligase
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Anti-SKP2 antibody (ab68455)

  • Datasheet
  • SDS
Reviews (1)Q&A (3)References (13)

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Western blot - Anti-SKP2 antibody (ab68455)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKP2 antibody (ab68455)
  • Western blot - Anti-SKP2 antibody (ab68455)

Key features and details

  • Rabbit polyclonal to SKP2
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SKP2 antibody
    See all SKP2 primary antibodies
  • Description

    Rabbit polyclonal to SKP2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human SKP2 aa 2-20 (N terminal). Different from the related rat and mouse sequences by three amino acids.
    Sequence:

    HRKHLQEIPDLSSNVATSF


    Database link: Q13309
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • F Box E3 Ligase
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Other
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human SKP2 protein (ab114385)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab68455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.
IHC-P
Use a concentration of 1 - 2 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.
IHC-P
Use a concentration of 1 - 2 µg/ml.

Target

  • Function

    Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1A, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 F-box domain.
    Contains 9 LRR (leucine-rich) repeats.
  • Target information above from: UniProt accession Q13309 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6502 Human
    • Omim: 601436 Human
    • SwissProt: Q13309 Human
    • Unigene: 23348 Human
    • Alternative names

      • CDK2/Cyclin A associated protein p45 antibody
      • Cyclin A/CDK2 associated protein p45 antibody
      • Cyclin-A/CDK2-associated protein p45 antibody
      • F box protein Skp2 antibody
      • F box/LRR repeat protein 1 antibody
      • F-box protein Skp2 antibody
      • F-box/LRR-repeat protein 1 antibody
      • FBL 1 antibody
      • FBL1 antibody
      • FBXL 1 antibody
      • FBXL1 antibody
      • FLB 1 antibody
      • FLB1 antibody
      • MGC1366 antibody
      • p45 antibody
      • p45skp2 antibody
      • S phase kinase associated protein 2 (p45) antibody
      • S phase kinase associated protein 2 antibody
      • S-phase kinase-associated protein 2 antibody
      • S-phase kinase-associated protein 2 E3 ubiquitin protein ligase antibody
      • SKP 2 antibody
      • Skp2 antibody
      • SKP2_HUMAN antibody
      see all

    Images

    • Western blot - Anti-SKP2 antibody (ab68455)
      Western blot - Anti-SKP2 antibody (ab68455)
      All lanes : Anti-SKP2 antibody (ab68455) at 1 µg/ml

      Lane 1 : Whole cell lysates prepared from Hela cells
      Lane 2 : Whole cell lysates prepared from 6T-CEM cells

      Lysates/proteins at 50 µg per lane.

      Secondary
      All lanes : HRP conjugated Goat anti-rabbit IgG at 1/3000 dilution

      Predicted band size: 48 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKP2 antibody (ab68455)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SKP2 antibody (ab68455)

      ab68455 at 1µg/ml staining SKP2 in Human mammary cancer tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). The tissue underwent enzymatic antigen retrieval in pepsin and trypsin. A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.

    • Western blot - Anti-SKP2 antibody (ab68455)
      Western blot - Anti-SKP2 antibody (ab68455)
      All lanes : Anti-SKP2 antibody (ab68455) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 3 : Human placenta tissue lysate - total protein (ab29745)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 48 kDa
      Observed band size: 53 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 29 kDa, 38 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 1 minute


      The predicted molecular weight of SKP2 is 48 kDa (SwissProt), however we expect to observe a banding pattern at 53 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (13)

    Publishing research using ab68455? Please let us know so that we can cite the reference in this datasheet.

    ab68455 has been referenced in 13 publications.

    • Qiu C  et al. Bisdemethoxycurcumin Inhibits Hepatocellular Carcinoma Proliferation Through Akt Inactivation via CYLD-Mediated Deubiquitination. Drug Des Devel Ther 14:993-1001 (2020). PubMed: 32184568
    • Zhang X  et al. IMP3 accelerates the progression of prostate cancer through inhibiting PTEN expression in a SMURF1-dependent way. J Exp Clin Cancer Res 39:190 (2020). PubMed: 32938489
    • Yang Y  et al. Skp2 inhibitor SKPin C1 decreased viability and proliferation of multiple myeloma cells and induced apoptosis. Braz J Med Biol Res 52:e8412 (2019). PubMed: 31038581
    • Yan W  et al. Inhibition of SKP2 Activity Impaired ATM-Mediated DNA Repair and Enhanced Sensitivity of Cisplatin-Resistant Mantle Cell Lymphoma Cells. Cancer Biother Radiopharm N/A:N/A (2019). PubMed: 31025879
    • He Z  et al. MicroRNA-186 targets SKP2 to induce p27Kip1-mediated pituitary tumor cell cycle deregulation and modulate cell proliferation. Korean J Physiol Pharmacol 23:171-179 (2019). PubMed: 31080348
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-SKP2 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (Amersham ECL Gel 8-16%)
    Sample
    Chinese Hamster Cell lysate - whole cell (CHO DG44 cell line)
    Specification
    CHO DG44 cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 16 2014

    Question


    we would like to test in WB the polycolonal ab19877.
    If you could send us a vial here is the address:

    Read More

    Abcam community

    Verified customer

    Asked on Apr 16 2012

    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab19877.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Apr 16 2012

    Question


    thanks for your reply. After consulting with people in the lab we were wondering if you could send us a sample of both your antibodies so that we can test in our cell model.
    Let me know if this is possible. In case we have to pick one is there a way you can tell us which one most likely would recognize the murine form?
    Thanks again

    Read More

    Abcam community

    Verified customer

    Asked on Apr 13 2012

    Answer

    Thank you for your reply.

    We do not have small test samples available to send.

    I would say both ab19877 and ab68887 have an equal likelihood of reacting with murine samples as both have similar percentage identity to the immunogens used to produce those antibodies.

    Please let me know which antibody you would like and I'll make sure to send it out right away.

    Read More

    Abcam Scientific Support

    Answered on Apr 13 2012

    Question

    .
    I am writing in reference of the skp2 antibody ab68544 that we have recently purchased by you with .
    We were unable to detect a specific band in protein lysates from mouse smooth muscle cells, our primary cell model. Therefore we tested in MEFs wild type versus MEFs Skp2 null (negative control), see attachment.
    As you can see there are no differences.

    Could we exchange with another skp2 antibody that works successfully in Western Blot analysis of mouse cell protein extracts?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 12 2012

    Answer

    Thank you for your enquiry and for including the western blotting data.

    I am sorry to see this vial of ab68455 is not working as expected. I would be happy to send you another vial of a different anti-SKP2 antibody. At this time none of our other SKP2 antibodies have been tested for reactivity to mouse SKP2.

    However, two antibodies to this protein that have a good chance of cross reacting with mouse SKP2, based on comparison of immunogen sequence with that of mouse, are ab19877 and ab68887. I would be happy to send either of these antibodies to you free of charge to test in your system.

    I look forward to hearing from you.

    Read More

    Abcam Scientific Support

    Answered on Apr 12 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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