• Product name

  • Description

    Rabbit polyclonal to SKP2
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide raised within aa: 1-50 of human SKP2.



Our Abpromise guarantee covers the use of ab87809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP Use at 10-15 µg/mg of lysate.


  • Function

    Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1A, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 F-box domain.
    Contains 9 LRR (leucine-rich) repeats.
  • Information by UniProt
  • Database links

  • Alternative names

    • CDK2/Cyclin A associated protein p45 antibody
    • Cyclin A/CDK2 associated protein p45 antibody
    • Cyclin-A/CDK2-associated protein p45 antibody
    • F box protein Skp2 antibody
    • F box/LRR repeat protein 1 antibody
    • F-box protein Skp2 antibody
    • F-box/LRR-repeat protein 1 antibody
    • FBL 1 antibody
    • FBL1 antibody
    • FBXL 1 antibody
    • FBXL1 antibody
    • FLB 1 antibody
    • FLB1 antibody
    • MGC1366 antibody
    • p45 antibody
    • p45skp2 antibody
    • S phase kinase associated protein 2 (p45) antibody
    • S phase kinase associated protein 2 antibody
    • S-phase kinase-associated protein 2 antibody
    • S-phase kinase-associated protein 2 E3 ubiquitin protein ligase antibody
    • SKP 2 antibody
    • Skp2 antibody
    • SKP2_HUMAN antibody
    see all


  • Lane 1: ab87809 (1µg/ml) immunoprecipitating SKP2 in HeLa Whole Cell Lysate (1mg for IP, 20% loaded).
    Lane 2: positive control IP (done using an antibody that recognises downstream epitope).
    Lane 3: Control IgG
  • All lanes : Anti-SKP2 antibody (ab87809) at 0.1 µg/ml

    Lane 1 : HeLa Whole Cell Lysate at 50 µg
    Lane 2 : HeLa Whole Cell Lysate at 15 µg
    Lane 3 : HeLa Whole Cell Lysate at 5 µg

    Predicted band size: 48 kDa


This product has been referenced in:

  • Li X  et al. SPAG9 regulates HEF1 expression and drives EMT in bladder transitional cell carcinoma via rac1 signaling pathway. Am J Cancer Res 8:2467-2480 (2018). Read more (PubMed: 30662804) »
See 1 Publication for this product

Customer reviews and Q&As


Thank you for contacting us.

The anti-SKP2 antibody (ab87809) has been used with HeLa whole cell lysate in order to detect the SKP2 protein. If your customer would like to purchase this as a pre-prepared lysate this can be purchased under the catalogue number https://www.abcam.com/HeLa-Human-epithelial-carcinoma-cell-line-Whole-Cell-Lysate-ab29545.html.

If your customer would like for me to have a look at the procedure they have been using in order to try and help resolve the problems I would be happy to try and help. If they could fill out the questionnaire I have attached to this email I will see if there are any further suggestions I can make.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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