Key features and details
- Rabbit polyclonal to SKP2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SKP2 antibody
See all SKP2 primary antibodies
DescriptionRabbit polyclonal to SKP2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide raised within aa: 1-50 of human SKP2.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab87809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
|IP||Use at 10-15 µg/mg of lysate.|
FunctionSubstrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1A, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 F-box domain.
Contains 9 LRR (leucine-rich) repeats.
- Information by UniProt
- CDK2/Cyclin A associated protein p45 antibody
- Cyclin A/CDK2 associated protein p45 antibody
- Cyclin-A/CDK2-associated protein p45 antibody
Lane 1: ab87809 (1µg/ml) immunoprecipitating SKP2 in HeLa Whole Cell Lysate (1mg for IP, 20% loaded).
Lane 2: positive control IP (done using an antibody that recognises downstream epitope).
Lane 3: Control IgG
All lanes : Anti-SKP2 antibody (ab87809) at 0.1 µg/ml
Lane 1 : HeLa Whole Cell Lysate at 50 µg
Lane 2 : HeLa Whole Cell Lysate at 15 µg
Lane 3 : HeLa Whole Cell Lysate at 5 µg
Predicted band size: 48 kDa
ab87809 has been referenced in 1 publication.
- Li X et al. SPAG9 regulates HEF1 expression and drives EMT in bladder transitional cell carcinoma via rac1 signaling pathway. Am J Cancer Res 8:2467-2480 (2018). PubMed: 30662804