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Dear technical team,
Our customer purchased this antibody and has difficulty to optimize the condition.
So, the customer would like to knowthe positive control when you had QC test.
Please let me know.
Asked on Jun 14 2012
Thank you for contacting us.
The anti-SKP2 antibody (ab87809) has been used with HeLa whole cell lysate in order to detect the SKP2 protein. If your customer would like to purchase this as a pre-prepared lysate this can be purchased under the catalogue number https://www.abcam.com/HeLa-Human-epithelial-carcinoma-cell-line-Whole-Cell-Lysate-ab29545.html.
If your customer would like for me to have a look at the procedure they have been using in order to try and help resolve the problems I would be happy to try and help. If they could fill out the questionnaire I have attached to this email I will see if there are any further suggestions I can make.
I hope this information has been of help. If you require any further information please do not hesitate to contact us again.
Answered on Jun 14 2012