Product nameAnti-SKP2 antibody [EPR3305(2)]
See all SKP2 primary antibodies
DescriptionRabbit monoclonal [EPR3305(2)] to SKP2
Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human SKP2 aa 1-100. The exact sequence is proprietary.
Database link: Q13309
- WB: C6, NIH/3T3, F9, A549, MDA-MB-231, Jurkat, MCF7, HepG2, and NCCIT cell lysates. ICC: 293T cells, FC: HeLa cells, IHC-P: Human kidney clear cell carcinoma tissue
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab183039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/200. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
FunctionSubstrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1A, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 F-box domain.
Contains 9 LRR (leucine-rich) repeats.
- Information by UniProt
- CDK2/Cyclin A associated protein p45 antibody
- Cyclin A/CDK2 associated protein p45 antibody
- Cyclin-A/CDK2-associated protein p45 antibody
Immunofluorescenct analysis of 4% paraformaldehyde fixed 293T cells labeling SKP2 with ab183039 at 1/100 followed by Goat anti rabbit IgG(Alexa Fluor® 488) at 1/200 (green). Cells were counter stained with Dapi (blue).
Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling SKP2 with ab183039 at 1/70 followed by Goat anti rabbit IgG (FITC) at 1/150. Rabbit monoclonal IgG was used as Isotype control.
All lanes : Anti-SKP2 antibody [EPR3305(2)] (ab183039) at 1/200 dilution
Lane 1 : NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
Lane 4 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates with 5% NFDM/TBST
Lane 5 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
Lane 6 : A549 (Human lung carcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
Lane 7 : F9 (Mouse embryonal carcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
Lane 8 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates with 5% NFDM/TBST
Lane 9 : C6 (Rat glial tumor glial cell) whole cell lysate with 5% NFDM/TBST
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 48 kDa
Exposure time: 180 seconds
We recommend to increase the amount of samples or decrease antibody dilution to get clear bands.
immunohistochemical analysis of paraffin embedded Human kidney clear cell carcinoma tissue labeling SKP2 with ab183039 at 1/50 followed by secondary staining with Ready to use HRP Polymer for Rabbit IgG and counterstained with Hematoxylin.
This product has been referenced in:
- Song J et al. Rottlerin promotes autophagy and apoptosis in gastric cancer cell lines. Mol Med Rep 18:2905-2913 (2018). Read more (PubMed: 30015872) »
- Dai Y et al. Activation of anaphase-promoting complex by p53 induces a state of dormancy in cancer cells against chemotherapeutic stress. Oncotarget 7:25478-92 (2016). WB . Read more (PubMed: 27009858) »