Overview

  • Product name

    Anti-SKP2 antibody [EPR3306(2)]
    See all SKP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR3306(2)] to SKP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human SKP2 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • 293T, HeLa, SW480 and LnCaP cell lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 46, 48 kDa (predicted molecular weight: 46, 48 kDa).
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1A, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Contains 1 F-box domain.
      Contains 9 LRR (leucine-rich) repeats.
    • Information by UniProt
    • Database links

    • Alternative names

      • CDK2/Cyclin A associated protein p45 antibody
      • Cyclin A/CDK2 associated protein p45 antibody
      • Cyclin-A/CDK2-associated protein p45 antibody
      • F box protein Skp2 antibody
      • F box/LRR repeat protein 1 antibody
      • F-box protein Skp2 antibody
      • F-box/LRR-repeat protein 1 antibody
      • FBL 1 antibody
      • FBL1 antibody
      • FBXL 1 antibody
      • FBXL1 antibody
      • FLB 1 antibody
      • FLB1 antibody
      • MGC1366 antibody
      • p45 antibody
      • p45skp2 antibody
      • S phase kinase associated protein 2 (p45) antibody
      • S phase kinase associated protein 2 antibody
      • S-phase kinase-associated protein 2 antibody
      • S-phase kinase-associated protein 2 E3 ubiquitin protein ligase antibody
      • SKP 2 antibody
      • Skp2 antibody
      • SKP2_HUMAN antibody
      see all

    Images

    • All lanes : Anti-SKP2 antibody [EPR3306(2)] (ab124799) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : SKP2 knockout HAP1 whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : MCF7 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 46, 48 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab124799 observed at 52 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab124799 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in SKP2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SKP2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab124799 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-SKP2 antibody [EPR3306(2)] (ab124799) at 1/1000 dilution

      Lane 1 : 293T cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : SW480 cell lysates
      Lane 4 : LnCaP cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 46, 48 kDa

    • ab124799, at a 1/50 dilution, staining SKP2 in HeLa cells by Immunofluorescence.

    References

    This product has been referenced in:

    • Yang B  et al. H2A.Z regulates tumorigenesis, metastasis and sensitivity to cisplatin in intrahepatic cholangiocarcinoma. Int J Oncol 52:1235-1245 (2018). Read more (PubMed: 29532867) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    No
    Specification
    HeLa
    Fixative
    Methanol

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Jan 04 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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