Key features and details
- Rat monoclonal [9D1] to SLAM / CD150 - Low endotoxin, Azide free
- Suitable for: Functional Studies, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG1
Product nameAnti-SLAM / CD150 antibody [9D1] - Low endotoxin, Azide free
See all SLAM / CD150 primary antibodies
DescriptionRat monoclonal [9D1] to SLAM / CD150 - Low endotoxin, Azide free
Tested applicationsSuitable for: Functional Studies, Flow Cytmore details
Species reactivityReacts with: Mouse
The details of the immunogen for this antibody are not available.
- Mouse thymic and splenic cell suspensions (C57Bl/6 splenocytes).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab174599 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.
Clone 9D1 has been reported to augment Tcell activation including inducing their proliferation and production of IFN-γ.
|Flow Cyt||Use 0.5-1µg for 105-8 cells.
(Final test volume = 100 µL).
ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionHigh-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates.
Tissue specificityConstitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
DomainThe most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues.
modificationsPhosphorylated by FYN.
Cellular localizationCell membrane. Present on the surface of B-cells and T-cells.
- Information by UniProt
- 4933415F16 antibody
- AA177906 antibody
- CD 150 antibody
ab174599 has not yet been referenced specifically in any publications.