RecombinantRabMAb

Recombinant Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)

Submit a review Submit a question

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22904-135] to SLAM / CD150
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-SLAM / CD150 antibody [EPR22904-135]
    See all SLAM / CD150 primary antibodies

  • Description

    Rabbit monoclonal [EPR22904-135] to SLAM / CD150

  • Host species

    Rabbit

  • Specificity

    IHC-P is recommended for mouse only.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr or IP

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Mouse SLAM/ CD150 aa 1-250. The exact sequence is proprietary.
    Database link: Q9QUM4

  • Positive control

    • WB: Mouse thymus and spleen lysates; rat thymus lysate. IHC-P Mouse spleen and bone marrow tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab228978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 38 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Recommended for mouse only.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr or IP.

    • Target

    • Function

      High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates.

    • Tissue specificity

      Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation.

    • Sequence similarities

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Domain

      The most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues.

    • Post-translational
      modifications

      Phosphorylated by FYN.

    • Cellular localization

      Cell membrane. Present on the surface of B-cells and T-cells.
    • Information by UniProt

    • Database links

      • Alternative names

        • 4933415F16 antibody
        • AA177906 antibody
        • CD 150 antibody
        • CD150 antibody
        • CD150 antigen antibody
        • CDw150 antibody
        • Estm51 antibody
        • Ipo 3 antibody
        • IPO-3 antibody
        • Ipo3 antibody
        • MGC151472 antibody
        • MGC151476 antibody
        • OTTHUMP00000025670 antibody
        • OTTHUMP00000060252 antibody
        • RGD1560634 antibody
        • Signaling lymphocytic activation molecule antibody
        • Signaling lymphocytic activation molecule family member 1 antibody
        • Signaling lymphocytic activation molecule family member 1, isoform CRA_a antibody
        • Signaling lymphocytic activation molecule family member 1, isoform CRA_b antibody
        • Signaling lymphocytic activation molecule precursor antibody
        • Slaf1 antibody
        • SLAF1_HUMAN antibody
        • SLAM family, member 1 antibody
        • Slamf 1 antibody
        • SLAMF1 antibody
        • SLAMF1 protein antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)

        Immunohistochemical analysis of paraffin-embedded mouse bone marrow tissue labeling SLAM /CD150 with ab228978 at 1/500 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab228978 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Positive staining on megakaryocytes in mouse bone marrow (PMID: 15989959) is observed. Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

         

         

      • Western blot - Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)
        Western blot - Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)
        All lanes : Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978) at 1/1000 dilution

        Lane 1 : Mouse thymus lysate
        Lane 2 : Mouse spleen lysate
        Lane 3 : Mouse lung lysate
        Lane 4 : Rat thymus lysate
        Lane 5 : Rat lung lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 38 kDa
        Observed band size: 75 kDa
        why is the actual band size different from the predicted?



        Blocking and dilution buffer: 5% NFDM/TBST.

        Exposure times.

        Lane 1: 26 seconds; Lanes 2-5: 136 seconds.

        The molecular weight observed is consistent with what has been described in the literature (PMID: 28982149).

        Negative control: Lung (PMID: 11950898)

         

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)

        Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling SLAM /CD150 with ab228978 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab228978 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Positive staining on megakaryocytes in mouse bone marrow (PMID: 15989959) is observed. Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

         

         

      • Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)
        Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab228978? Please let us know so that we can cite the reference in this datasheet.

      ab228978 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab228978.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com