Recombinant
RabMAb

Recombinant Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)

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Overview

  • Product name

    Anti-SLAM / CD150 antibody [EPR22904-135]
    See all SLAM / CD150 primary antibodies

  • Description

    Rabbit monoclonal [EPR22904-135] to SLAM / CD150

  • Host species

    Rabbit

  • Specificity

    IHC-P is recommended for mouse only.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr or IP

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Mouse SLAM/ CD150 aa 1-250. The exact sequence is proprietary.
    Database link: Q9QUM4

  • Positive control

    • WB: Mouse thymus and spleen lysates; rat thymus lysate. IHC-P Mouse spleen and bone marrow tissue.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab228978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 38 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Recommended for mouse only.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr or IP.

    • Target

    • Function

      High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates.

    • Tissue specificity

      Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation.

    • Sequence similarities

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Domain

      The most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues.

    • Post-translational
      modifications

      Phosphorylated by FYN.

    • Cellular localization

      Cell membrane. Present on the surface of B-cells and T-cells.
    • Information by UniProt

    • Database links

      • Alternative names

        • 4933415F16 antibody
        • AA177906 antibody
        • CD 150 antibody
        • CD150 antibody
        • CD150 antigen antibody
        • CDw150 antibody
        • Estm51 antibody
        • Ipo 3 antibody
        • IPO-3 antibody
        • Ipo3 antibody
        • MGC151472 antibody
        • MGC151476 antibody
        • OTTHUMP00000025670 antibody
        • OTTHUMP00000060252 antibody
        • RGD1560634 antibody
        • Signaling lymphocytic activation molecule antibody
        • Signaling lymphocytic activation molecule family member 1 antibody
        • Signaling lymphocytic activation molecule family member 1, isoform CRA_a antibody
        • Signaling lymphocytic activation molecule family member 1, isoform CRA_b antibody
        • Signaling lymphocytic activation molecule precursor antibody
        • Slaf1 antibody
        • SLAF1_HUMAN antibody
        • SLAM family, member 1 antibody
        • Slamf 1 antibody
        • SLAMF1 antibody
        • SLAMF1 protein antibody
        see all

      Images

      • Western blot - Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)
        Western blot - Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)
        All lanes : Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978) at 1/1000 dilution

        Lane 1 : Mouse thymus lysate
        Lane 2 : Mouse spleen lysate
        Lane 3 : Mouse lung lysate
        Lane 4 : Rat thymus lysate
        Lane 5 : Rat lung lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 38 kDa
        Observed band size: 75 kDa
        why is the actual band size different from the predicted?



        Blocking and dilution buffer: 5% NFDM/TBST.

        Exposure times.

        Lane 1: 26 seconds; Lanes 2-5: 136 seconds.

        The molecular weight observed is consistent with what has been described in the literature (PMID: 28982149).

        Negative control: Lung (PMID: 11950898)

         

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)

        Immunohistochemical analysis of paraffin-embedded mouse bone marrow tissue labeling SLAM /CD150 with ab228978 at 1/500 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab228978 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Positive staining on megakaryocytes in mouse bone marrow (PMID: 15989959) is observed. Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

         

         

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)

        Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling SLAM /CD150 with ab228978 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab228978 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Positive staining on megakaryocytes in mouse bone marrow (PMID: 15989959) is observed. Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

         

         

      References

      ab228978 has not yet been referenced specifically in any publications.

      Publishing research using ab228978? Please let us know so that we can cite the reference in this datasheet.

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