Product nameAnti-SLAM / CD150 antibody [EPR5470]
See all SLAM / CD150 primary antibodies
DescriptionRabbit monoclonal [EPR5470] to SLAM / CD150
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human SLAM/ CD150 aa 250-350. The exact sequence is proprietary.
- WB: Jurkat, HuT 78, MOLT4, Daudi and human thymus lysates. Flow Cyt: Raji cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 85 kDa.|
|IP||1/10 - 1/100.|
|Flow Cyt||Use at an assay dependent concentration.|
FunctionHigh-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates.
Tissue specificityConstitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
DomainThe most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues.
modificationsPhosphorylated by FYN.
Cellular localizationCell membrane. Present on the surface of B-cells and T-cells.
- Information by UniProt
- 4933415F16 antibody
- AA177906 antibody
- CD 150 antibody
All lanes : Anti-SLAM / CD150 antibody [EPR5470] (ab134044) at 1/1000 dilution
Lane 1 : Jurkat lysate
Lane 2 : HuT 78 lysate
Lane 3 : MOLT4 lysate
Lane 4 : Daudi lysate
Lane 5 : Human thymus lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 85 kDa
Flow cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling SLAM / CD150 with ab134044 at 1/70 dilution, (11.13 µg/ml) (red). Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488, Ab150077) was used as the secondary antibody at 1:2000. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabeled cells (Blue).
ab134044 has not yet been referenced specifically in any publications.