For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    slam--cd150-antibody-epr5470-ab134044.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Cell Type Markers CD Non-lineage
Share by email
RecombinantRabMAb

Recombinant Anti-SLAM / CD150 antibody [EPR5470] (ab134044)

  • Datasheet
  • SDS
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-SLAM / CD150 antibody [EPR5470] (ab134044)
  • Flow Cytometry - Anti-SLAM / CD150 antibody [EPR5470] (ab134044)
  • Anti-SLAM / CD150 antibody [EPR5470] (ab134044)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5470] to SLAM / CD150
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-SLAM / CD150 antibody [EPR5470]
    See all SLAM / CD150 primary antibodies
  • Description

    Rabbit monoclonal [EPR5470] to SLAM / CD150
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SLAM/ CD150 aa 250-350. The exact sequence is proprietary.

  • Positive control

    • WB: Jurkat, HuT 78, MOLT4, Daudi and human thymus lysates. Flow Cyt: Raji cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5470
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Non-lineage
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • HSC markers
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Alternative Versions

    • Anti-SLAM / CD150 antibody [EPR5470] - BSA and Azide free (ab246710)
    • Anti-SLAM / CD150 antibody [EPR5470] - Low endotoxin, Azide free (ab246792)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat membrane extract lysate (ab30130)
  • Recombinant Protein

    • Recombinant Human SLAM / CD150 protein (denatured) (ab139259)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 85 kDa.
Flow Cyt
Use at an assay dependent concentration.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 85 kDa.
Flow Cyt
Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function

      High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates.
    • Tissue specificity

      Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation.
    • Sequence similarities

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Domain

      The most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues.
    • Post-translational
      modifications

      Phosphorylated by FYN.
    • Cellular localization

      Cell membrane. Present on the surface of B-cells and T-cells.
    • Target information above from: UniProt accession Q13291 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6504 Human
      • Omim: 603492 Human
      • SwissProt: Q13291 Human
      • Unigene: 523660 Human
      • Alternative names

        • 4933415F16 antibody
        • AA177906 antibody
        • CD 150 antibody
        • CD150 antibody
        • CD150 antigen antibody
        • CDw150 antibody
        • Estm51 antibody
        • Ipo 3 antibody
        • IPO-3 antibody
        • Ipo3 antibody
        • MGC151472 antibody
        • MGC151476 antibody
        • OTTHUMP00000025670 antibody
        • OTTHUMP00000060252 antibody
        • RGD1560634 antibody
        • Signaling lymphocytic activation molecule antibody
        • Signaling lymphocytic activation molecule family member 1 antibody
        • Signaling lymphocytic activation molecule family member 1, isoform CRA_a antibody
        • Signaling lymphocytic activation molecule family member 1, isoform CRA_b antibody
        • Signaling lymphocytic activation molecule precursor antibody
        • Slaf1 antibody
        • SLAF1_HUMAN antibody
        • SLAM family, member 1 antibody
        • Slamf 1 antibody
        • SLAMF1 antibody
        • SLAMF1 protein antibody
        see all

      Images

      • Western blot - Anti-SLAM / CD150 antibody [EPR5470] (ab134044)
        Western blot - Anti-SLAM / CD150 antibody [EPR5470] (ab134044)
        All lanes : Anti-SLAM / CD150 antibody [EPR5470] (ab134044) at 1/1000 dilution

        Lane 1 : Jurkat lysate
        Lane 2 : HuT 78 lysate
        Lane 3 : MOLT4 lysate
        Lane 4 : Daudi lysate
        Lane 5 : Human thymus lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 85 kDa

      • Flow Cytometry - Anti-SLAM / CD150 antibody [EPR5470] (ab134044)
        Flow Cytometry - Anti-SLAM / CD150 antibody [EPR5470] (ab134044)

        Flow cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling SLAM / CD150 with ab134044 at 1/70 dilution, (11.13 µg/ml)  (red). Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488, Ab150077) was used as the secondary antibody at 1:2000. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control -  Unlabeled cells (Blue).

      • Anti-SLAM / CD150 antibody [EPR5470] (ab134044)
        Anti-SLAM / CD150 antibody [EPR5470] (ab134044)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab134044? Please let us know so that we can cite the reference in this datasheet.

      ab134044 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab134044.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.