Recombinant Anti-SLAM / CD150 antibody [EPR5470] (ab134044)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-SLAM / CD150 antibody [EPR5470]
See all SLAM / CD150 primary antibodies -
Description
Rabbit monoclonal [EPR5470] to SLAM / CD150 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human SLAM/ CD150 aa 250-350. The exact sequence is proprietary.
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Positive control
- WB: Jurkat, HuT 78, MOLT4, Daudi and human thymus lysates. Flow Cyt: Raji cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5470 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab134044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 85 kDa. | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | Use at an assay dependent concentration. |
Target
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Function
High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates. -
Tissue specificity
Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Domain
The most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues. -
Post-translational
modificationsPhosphorylated by FYN. -
Cellular localization
Cell membrane. Present on the surface of B-cells and T-cells. - Information by UniProt
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Database links
- Entrez Gene: 6504 Human
- Omim: 603492 Human
- SwissProt: Q13291 Human
- Unigene: 523660 Human
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Alternative names
- 4933415F16 antibody
- AA177906 antibody
- CD 150 antibody
see all
Images
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![Western blot - Anti-SLAM / CD150 antibody [EPR5470] (ab134044)](https://www.abcam.com/ps/products/134/ab134044/Images/ab134044-148140-anti-slam-cd150-antibody-epr5470-western-blot.jpg)
Western blot - Anti-SLAM / CD150 antibody [EPR5470] (ab134044)All lanes : Anti-SLAM / CD150 antibody [EPR5470] (ab134044) at 1/1000 dilution
Lane 1 : Jurkat lysate
Lane 2 : HuT 78 lysate
Lane 3 : MOLT4 lysate
Lane 4 : Daudi lysate
Lane 5 : Human thymus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 85 kDa -
![Flow Cytometry - Anti-SLAM / CD150 antibody [EPR5470] (ab134044)](https://www.abcam.com/ps/products/134/ab134044/Images/ab134044-299356-anti-slam-cd150-antibody-epr5470-flow-cytometry.jpg)
Flow Cytometry - Anti-SLAM / CD150 antibody [EPR5470] (ab134044)Flow cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling SLAM / CD150 with ab134044 at 1/70 dilution, (11.13 µg/ml) (red). Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488, Ab150077) was used as the secondary antibody at 1:2000. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabeled cells (Blue).
Datasheets and documents
References
ab134044 has not yet been referenced specifically in any publications.
