Recombinant Anti-SLAM / CD150 antibody [EPR5470] - BSA and Azide free (ab246710)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5470] to SLAM / CD150 - BSA and Azide free
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SLAM / CD150 antibody [EPR5470] - BSA and Azide free
See all SLAM / CD150 primary antibodies -
Description
Rabbit monoclonal [EPR5470] to SLAM / CD150 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat, HuT 78, MOLT4, Daudi and human thymus lysates. Flow Cyt (intra): Raji cells.
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General notes
ab246710 is the carrier-free version of ab134044.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5470 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-SLAM / CD150 antibody [EPR5470] (ab134044)
- Anti-SLAM / CD150 antibody [EPR5470] - Low endotoxin, Azide free (ab246792)
- Alexa Fluor® 488 Anti-SLAM / CD150 antibody [EPR5470] (ab309911)
- Alexa Fluor® 647 Anti-SLAM / CD150 antibody [EPR5470] (ab310278)
- Alexa Fluor® 594 Anti-SLAM / CD150 antibody [EPR5470] (ab310732)
- Alexa Fluor® 555 Anti-SLAM / CD150 antibody [EPR5470] (ab312262)
- Alexa Fluor® 568 Anti-SLAM / CD150 antibody [EPR5470] (ab312751)
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab246710 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 85 kDa.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 85 kDa. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
Target
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Function
High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates. -
Tissue specificity
Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Domain
The most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues. -
Post-translational
modificationsPhosphorylated by FYN. -
Cellular localization
Cell membrane. Present on the surface of B-cells and T-cells. - Information by UniProt
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Database links
- Entrez Gene: 6504 Human
- Omim: 603492 Human
- SwissProt: Q13291 Human
- Unigene: 523660 Human
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Alternative names
- 4933415F16 antibody
- AA177906 antibody
- CD 150 antibody
see all
Images
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Intracellular Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling SLAM / CD150 with ab134044 at 1/70 dilution, (11.13µg/ml) (red). Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488, Ab150077) was used as the secondary antibody at 1/2000. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabeled cells (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing Tris Glycine, BSA, glycerol and sodium azide (ab134044).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab246710 has not yet been referenced specifically in any publications.