The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 4µl for 106 cells.
or 100 μl of whole blood
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates.
Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation.