Recombinant Anti-SLAMF6 antibody [EPR23122-130] (ab243658)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23122-130] to SLAMF6
- Suitable for: ICC/IF, Flow Cyt, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-SLAMF6 antibody [EPR23122-130]
See all SLAMF6 primary antibodies -
Description
Rabbit monoclonal [EPR23122-130] to SLAMF6 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IPmore details
Unsuitable for: IHC-P or WB -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: EL4 and mouse splenocytes cells. Flow Cyt: Mouse splenocytes cells. IP: Mouse spleen lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23122-130 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50.
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Flow Cyt |
1/500.
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IP |
1/30.
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Notes |
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ICC/IF
1/50. |
Flow Cyt
1/500. |
IP
1/30. |
Target
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Function
Triggers cytolytic activity only in natural killer cells (NK) expressing high surface densities of natural cytotoxicity receptors. -
Tissue specificity
Expressed by all (resting and activated) natural killer cells (NK), T- and B-lymphocytes. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 30925 Mouse
- SwissProt: Q9ET39 Mouse
- Unigene: 486404 Mouse
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Alternative names
- Activating NK receptor antibody
- CD352 antibody
- KALI antibody
see all
Images
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SLAMF6 was immunoprecipitated from 0.35 mg Mouse spleen lysate with ab243658 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243658 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: Mouse spleen lysate 10µg
Lane 2: ab243658 IP in Mouse spleen lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243658 in Mouse spleen lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 min
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Flow cytometric analysis of Mouse splenocytes cells labelling SLAMF6 with ab243658 at 1/500 compared with a Rabbit monoclonal IgG (ab172730, Left) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse splenocytes cells labelling SLAMF6 with ab243658 at 1/50 dilution, followed by ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing staining in some subsets of mouse splenocytes is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 dilution.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EL4 (mouse lymphoma T lymphocyte) cells labelling SLAMF6 with ab243658 at 1/50 dilution, followed by ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in EL4 cells is observed. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab243658 has not yet been referenced specifically in any publications.