Overview

  • Product name

    Anti-SLAMF7/CS1 antibody [5G5A4]
    See all SLAMF7/CS1 primary antibodies
  • Description

    Mouse monoclonal [5G5A4] to SLAMF7/CS1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human SLAMF7/CS1 aa 23-226. Extracellular domain. Expressed in E.coli.
    Database link: Q9NQ25

  • Positive control

    • WB: Recombinant human SLAMF7/CS1 (aa 23-226) protein; Human SLAMF7/CS1 (aa 23-226)-hIgG-Fc-transfected HEK-293 cell lysate. IHC-P: Human tonsil and rectum cancer tissue. Flow cyt: Raji cells.
  • General notes

     This product was previously labelled as SLAMF7

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    5G5A4
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab233779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 37 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

Target

  • Relevance

    SLAMF7 contains one Ig-like C2-type (immunoglobulin-like) domain. Isoform 1 mediates NK cell activation through a SAP-independent extracellular signal-regulated ERK-mediated pathway. It may play a role in lymphocyte adhesion. Isoform 3 does not mediate any activation. SAP can bind the cytoplasmic tail of isoform 1 when phosphorylated in the presence of Fyn (in vitro). SLAMF7 is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Expression was detected in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-, or T-cell lines. The isoform 3 is expressed at much lower level than isoform 1. There are three named isoforms.
  • Cellular localization

    Membrane; Single-pass type I membrane protein.
  • Database links

  • Alternative names

    • 19A antibody
    • 19A24 protein antibody
    • CD 319 antibody
    • CD2 like receptor activating cytotoxic cells antibody
    • CD2 subset 1 antibody
    • CD319 antibody
    • CD319 antigen antibody
    • CRACC antibody
    • CS1 antibody
    • Membrane protein FOAP 12 antibody
    • Novel Ly9 antibody
    • Novel LY9 (lymphocyte antigen 9) like protein antibody
    • Protein 19A antibody
    • SLAM family member 7 antibody
    • SLAMF 7 antibody
    see all

Images

  • Anti-SLAMF7/CS1 antibody [5G5A4] (ab233779) at 1/500 dilution + Recombinant human SLAMF7/CS1 (aa 23-226) protein

    Predicted band size: 37 kDa



    Expected MW is 48.3 kDa.

  • All lanes : Anti-SLAMF7/CS1 antibody [5G5A4] (ab233779) at 1/500 dilution

    Lane 1 : Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : Human SLAMF7/CS1 (aa 23-226)-hIgG-Fc-transfected HEK-293 cell lysate

    Predicted band size: 37 kDa

  • Flow cytometric analysis of Raji (human Burkitt's lymphoma cell line) cells labeling SLAMF7/CS1 with ab233779 at 1/200 dilution (green) compared with a negative control (red).

  • Paraffin-embedded human tonsil tissue stained for SLAMF7/CS1 using ab233779 at 1/200 dilution in immunohistochemical analysis. DAB staining.

  • Paraffin-embedded human rectum cancer tissue stained for SLAMF7/CS1 using ab233779 at 1/200 dilution in immunohistochemical analysis. DAB staining.

References

ab233779 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab233779.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up