Anti-SLAMF7/CS1 antibody [5G5A4] (ab233779)
Key features and details
- Mouse monoclonal [5G5A4] to SLAMF7/CS1
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-SLAMF7/CS1 antibody [5G5A4]
See all SLAMF7/CS1 primary antibodies -
Description
Mouse monoclonal [5G5A4] to SLAMF7/CS1 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human SLAMF7/CS1 aa 23-226. Extracellular domain. Expressed in E.coli.
Database link: Q9NQ25 -
Positive control
- WB: Recombinant human SLAMF7/CS1 (aa 23-226) protein; Human SLAMF7/CS1 (aa 23-226)-hIgG-Fc-transfected HEK-293 cell lysate. IHC-P: Human tonsil and rectum cancer tissue. Flow cyt: Raji cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
5G5A4 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 37 kDa.
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IHC-P |
1/200 - 1/1000.
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Flow Cyt |
1/200 - 1/400.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 37 kDa. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. |
Target
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Relevance
SLAMF7 contains one Ig-like C2-type (immunoglobulin-like) domain. Isoform 1 mediates NK cell activation through a SAP-independent extracellular signal-regulated ERK-mediated pathway. It may play a role in lymphocyte adhesion. Isoform 3 does not mediate any activation. SAP can bind the cytoplasmic tail of isoform 1 when phosphorylated in the presence of Fyn (in vitro). SLAMF7 is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Expression was detected in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-, or T-cell lines. The isoform 3 is expressed at much lower level than isoform 1. There are three named isoforms. -
Cellular localization
Membrane; Single-pass type I membrane protein. -
Database links
- Entrez Gene: 57823 Human
- Omim: 606625 Human
- SwissProt: Q9NQ25 Human
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Alternative names
- 19A antibody
- 19A24 protein antibody
- CD 319 antibody
see all
Images
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Anti-SLAMF7/CS1 antibody [5G5A4] (ab233779) at 1/500 dilution + Recombinant human SLAMF7/CS1 (aa 23-226) protein
Predicted band size: 37 kDaExpected MW is 48.3 kDa.
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All lanes : Anti-SLAMF7/CS1 antibody [5G5A4] (ab233779) at 1/500 dilution
Lane 1 : Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : Human SLAMF7/CS1 (aa 23-226)-hIgG-Fc-transfected HEK-293 cell lysate
Predicted band size: 37 kDa -
Flow cytometric analysis of Raji (human Burkitt's lymphoma cell line) cells labeling SLAMF7/CS1 with ab233779 at 1/200 dilution (green) compared with a negative control (red).
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Paraffin-embedded human tonsil tissue stained for SLAMF7/CS1 using ab233779 at 1/200 dilution in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human rectum cancer tissue stained for SLAMF7/CS1 using ab233779 at 1/200 dilution in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab233779 has not yet been referenced specifically in any publications.