Recombinant

Recombinant Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)

Submit a review Submit a question

Overview

  • Product name

    Anti-SLAMF7/CS1 antibody [CAL7]
    See all SLAMF7/CS1 primary antibodies

  • Description

    Rabbit monoclonal [CAL7] to SLAMF7/CS1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to Human SLAMF7/CS1 aa 248-335.
    Database link: Q9NQ25

  • Positive control

    • IHC-P: SLAMF7/CS1 Knock-in AD-293 cells; OPM2 xenograft tissue. WB: IM-9 cell lysate IP: Human tonsil and IM-9 lysate

  • General notes

    (aka clone 43H1L46)

     This product was previously labelled as SLAMF7

     

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.50
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Purity >99%

  • Clonality

    Monoclonal

  • Clone number

    CAL7

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab237730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
IP 1/30.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.

    • Target

    • Relevance

      SLAMF7 contains one Ig-like C2-type (immunoglobulin-like) domain. Isoform 1 mediates NK cell activation through a SAP-independent extracellular signal-regulated ERK-mediated pathway. It may play a role in lymphocyte adhesion. Isoform 3 does not mediate any activation. SAP can bind the cytoplasmic tail of isoform 1 when phosphorylated in the presence of Fyn (in vitro). SLAMF7 is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Expression was detected in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-, or T-cell lines. The isoform 3 is expressed at much lower level than isoform 1. There are three named isoforms.

    • Cellular localization

      Membrane; Single-pass type I membrane protein.

    • Database links

      • Alternative names

        • 19A antibody
        • 19A24 protein antibody
        • CD 319 antibody
        • CD2 like receptor activating cytotoxic cells antibody
        • CD2 subset 1 antibody
        • CD319 antibody
        • CD319 antigen antibody
        • CRACC antibody
        • CS1 antibody
        • Membrane protein FOAP 12 antibody
        • Novel Ly9 antibody
        • Novel LY9 (lymphocyte antigen 9) like protein antibody
        • Protein 19A antibody
        • SLAM family member 7 antibody
        • SLAMF 7 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)

        Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling SLAMF7/CS1 with ab237730 at 1/800 dilution for 15 minutes at room temperature, followed by ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on human tonsil is observed. Counterstained with Hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)

        Immunohistochemical analysis of paraffin-embedded human multiple myeloma tissue labelling SLAMF7/CS1 with ab237730 at 1/800 dilution for 15 minutes at room temperature, followed by ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly membranous staining on the human multiple myeloma is observed. Counterstained with Hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      • Western blot - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)
        Western blot - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)
        All lanes : Anti-SLAMF7/CS1 antibody [CAL7] (ab237730) at 1/1000 dilution

        All lanes : IM-9 (human multiple myeloma B Lymphoblast) whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution (VeriBlot for IP Detection Reagent (HRP))
        Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

        Predicted band size: 37 kDa



        Blocking/Diluting buffer and concentration: 5% NFDM/TBST

        Exposure time:
        Lane 1: 3 min
        Lane 2: 1min

        The expression profile observed is consistent with what has been described in the literature (PMID: 18451245; 11698418; 25312647), with the bands greater than 37 kDa predicted to be glycosylated SLAMF7/CS1.

      • Immunoprecipitation - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)
        Immunoprecipitation - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)

        SLAMF7/CS1 was immunoprecipitated from 0.35mg of Human tonsil lysate with ab237730 at 1/30 dilution. Western blot was performed from the immunoprecipitated using ab237730 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as secondary antibody at 1/5000 dilution.

        Lane 1: Human tonsil lysate 10μg (Input)

        Lane 2: ab237730 IP in Human tonsil lysate

        Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237730 in Human tonsil lysate

         

        Blocking/Diluting buffer and concentration: 5% NFDM/TBST

      • Immunoprecipitation - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)
        Immunoprecipitation - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)

        SLAMF7/CS1 was immunoprecipitated from 0.35mg of IM-9 (human multiple myeloma B Lymphoblast) whole cell lysate with ab237730 at 1/30 dilution. Western blot was performed from the immunoprecipitated using ab237730 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as secondary antibody at 1/5000 dilution.

        Lane 1: IM-9 (human multiple myeloma B Lymphoblast) whole cell lysate 10μg (Input)

        Lane 2: ab237730 IP in IM-9 lysate

        Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237730 in IM-9 whole cell lysate

        Blocking/diluting buffer and concentration: 5% NFDM/TBST

        The expression profile observed is consistent with what has been described in the literature (PMID: 18451245; 11698418; 25312647), with the bands greater than 37 kDa predicted to be glycosylated SLAMF7/CS1.

         

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)

        Formalin-fixed, paraffin-embedded multiple myeloma (Lambda, left panel) and SLAMF7 Knock-in AD-293 cells  (Positive control, right panel) stained for SLAMF7/CS1 using ab237730 at 0.1 µg/ml dilution in immunohistochemical analysis.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)

        Formalin-fixed, paraffin-embedded OPM2 xenograft (Postive control, left panel) and NCI-N87 xenograft (Negative control, right) tissue stained for SLAMF7/CS1 using ab237730 at 0.1 µg/ml dilution in immunohistochemical analysis.

      References

      ab237730 has not yet been referenced specifically in any publications.

      Publishing research using ab237730? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab237730.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com