Recombinant Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)](https://www.abcam.com/ps/products/237/ab237730/Images/ab237730-345779-anti-slamf7-cs1-antibody-cal7-immunohistochemistry-tonsil-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [CAL7] to SLAMF7/CS1
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-SLAMF7/CS1 antibody [CAL7]
See all SLAMF7/CS1 primary antibodies -
Description
Rabbit monoclonal [CAL7] to SLAMF7/CS1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human SLAMF7/CS1 aa 248-335.
Database link: Q9NQ25 -
Positive control
- IHC-P: SLAMF7/CS1 Knock-in AD-293 cells; OPM2 xenograft tissue. WB: IM-9 cell lysate IP: Human tonsil and IM-9 lysate
-
General notes
(aka clone 43H1L46)
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purity >99% -
Clonality
Monoclonal -
Clone number
CAL7 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237730 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| WB |
1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
|
|
| IP |
1/30.
|
| Notes |
|---|
|
IHC-P
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). |
|
IP
1/30. |
Target
-
Relevance
SLAMF7 contains one Ig-like C2-type (immunoglobulin-like) domain. Isoform 1 mediates NK cell activation through a SAP-independent extracellular signal-regulated ERK-mediated pathway. It may play a role in lymphocyte adhesion. Isoform 3 does not mediate any activation. SAP can bind the cytoplasmic tail of isoform 1 when phosphorylated in the presence of Fyn (in vitro). SLAMF7 is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Expression was detected in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-, or T-cell lines. The isoform 3 is expressed at much lower level than isoform 1. There are three named isoforms. -
Cellular localization
Membrane; Single-pass type I membrane protein. -
Database links
- Entrez Gene: 57823 Human
- Omim: 606625 Human
- SwissProt: Q9NQ25 Human
-
Alternative names
- 19A antibody
- 19A24 protein antibody
- CD 319 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling SLAMF7/CS1 with ab237730 at 1/800 dilution for 15 minutes at room temperature, followed by ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on human tonsil is observed. Counterstained with Hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)](https://www.abcam.com/ps/products/237/ab237730/Images/ab237730-345778-anti-slamf7-cs1-antibody-cal7-immunohistochemistry-multiple-myeloma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)Immunohistochemical analysis of paraffin-embedded human multiple myeloma tissue labelling SLAMF7/CS1 with ab237730 at 1/800 dilution for 15 minutes at room temperature, followed by ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly membranous staining on the human multiple myeloma is observed. Counterstained with Hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
-
![Western blot - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)](https://www.abcam.com/ps/products/237/ab237730/Images/ab237730-345780-anti-slamf7-cs1-antibody-cal7-western-blot-human.jpg)
Western blot - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)All lanes : Anti-SLAMF7/CS1 antibody [CAL7] (ab237730) at 1/1000 dilution
All lanes : IM-9 (human multiple myeloma B Lymphoblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution (VeriBlot for IP Detection Reagent (HRP))
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 37 kDaBlocking/Diluting buffer and concentration: 5% NFDM/TBST
Exposure time:
Lane 1: 3 min
Lane 2: 1minThe expression profile observed is consistent with what has been described in the literature (PMID: 18451245; 11698418; 25312647), with the bands greater than 37 kDa predicted to be glycosylated SLAMF7/CS1.
-
![Immunoprecipitation - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)](https://www.abcam.com/ps/products/237/ab237730/Images/ab237730-345782-anti-slamf7-cs1-antibody-cal7-immunoprecipitation-tonsil-human.jpg)
Immunoprecipitation - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)SLAMF7/CS1 was immunoprecipitated from 0.35mg of Human tonsil lysate with ab237730 at 1/30 dilution. Western blot was performed from the immunoprecipitated using ab237730 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as secondary antibody at 1/5000 dilution.
Lane 1: Human tonsil lysate 10μg (Input)
Lane 2: ab237730 IP in Human tonsil lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237730 in Human tonsil lysate
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
-
![Immunoprecipitation - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)](https://www.abcam.com/ps/products/237/ab237730/Images/ab237730-345781-anti-slamf7-cs1-antibody-cal7-immunoprecipitation-human.jpg)
Immunoprecipitation - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)SLAMF7/CS1 was immunoprecipitated from 0.35mg of IM-9 (human multiple myeloma B Lymphoblast) whole cell lysate with ab237730 at 1/30 dilution. Western blot was performed from the immunoprecipitated using ab237730 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as secondary antibody at 1/5000 dilution.
Lane 1: IM-9 (human multiple myeloma B Lymphoblast) whole cell lysate 10μg (Input)
Lane 2: ab237730 IP in IM-9 lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237730 in IM-9 whole cell lysate
Blocking/diluting buffer and concentration: 5% NFDM/TBST
The expression profile observed is consistent with what has been described in the literature (PMID: 18451245; 11698418; 25312647), with the bands greater than 37 kDa predicted to be glycosylated SLAMF7/CS1.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)](https://www.abcam.com/ps/products/237/ab237730/Images/ab237730-315304-anti-slamf7-antibody-cal7-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)Formalin-fixed, paraffin-embedded multiple myeloma (Lambda, left panel) and SLAMF7 Knock-in AD-293 cells (Positive control, right panel) stained for SLAMF7/CS1 using ab237730 at 0.1 µg/ml dilution in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)](https://www.abcam.com/ps/products/237/ab237730/Images/ab237730-315305-anti-slamf7-antibody-cal7-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)Formalin-fixed, paraffin-embedded OPM2 xenograft (Postive control, left panel) and NCI-N87 xenograft (Negative control, right) tissue stained for SLAMF7/CS1 using ab237730 at 0.1 µg/ml dilution in immunohistochemical analysis.
-
![Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)](https://www.abcam.com/ps/products/237/ab237730/Images/ab237730-8-benefits-of-recombinant-antibodies.png)
Anti-SLAMF7/CS1 antibody [CAL7] (ab237730)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (1)
ab237730 has been referenced in 1 publication.
- von Wenserski L et al. SLAMF receptors negatively regulate B cell receptor signaling in chronic lymphocytic leukemia via recruitment of prohibitin-2. Leukemia 35:1073-1086 (2021). PubMed: 32826957
