Recombinant
RabMAb

Recombinant Anti-SLAMF7/CS1 antibody [EPR21155] - BSA and Azide free (ab233090)

Overview

  • Product name

    Anti-SLAMF7/CS1 antibody [EPR21155] - BSA and Azide free
    See all SLAMF7/CS1 primary antibodies
  • Description

    Rabbit monoclonal [EPR21155] to SLAMF7/CS1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human SLAMF7/CS1 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9NQ25

  • Positive control

    • IHC-P: Human multiple myeloma tissue.
  • General notes

    ab233090 is the carrier-free version of ab230945 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab233090 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as SLAMF7

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21155
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab233090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Relevance

    SLAMF7 contains one Ig-like C2-type (immunoglobulin-like) domain. Isoform 1 mediates NK cell activation through a SAP-independent extracellular signal-regulated ERK-mediated pathway. It may play a role in lymphocyte adhesion. Isoform 3 does not mediate any activation. SAP can bind the cytoplasmic tail of isoform 1 when phosphorylated in the presence of Fyn (in vitro). SLAMF7 is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Expression was detected in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-, or T-cell lines. The isoform 3 is expressed at much lower level than isoform 1. There are three named isoforms.
  • Cellular localization

    Membrane; Single-pass type I membrane protein.
  • Database links

  • Alternative names

    • 19A antibody
    • 19A24 protein antibody
    • CD 319 antibody
    • CD2 like receptor activating cytotoxic cells antibody
    • CD2 subset 1 antibody
    • CD319 antibody
    • CD319 antigen antibody
    • CRACC antibody
    • CS1 antibody
    • Membrane protein FOAP 12 antibody
    • Novel Ly9 antibody
    • Novel LY9 (lymphocyte antigen 9) like protein antibody
    • Protein 19A antibody
    • SLAM family member 7 antibody
    • SLAMF 7 antibody
    see all

Images

  • SLAMF7/CS1 was immunoprecipitated from 0.35 mg of IM-9 (human multiple myeloma B lymphoblast cell line) whole cell lysate with ab230945 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230945 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: IM-9 whole cell lysate 10 μg (Input). 

    Lane 2: ab230945 IP in IM-9 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230945 in IM-9 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 seconds.

    The expression profile observed is consistent with what has been described in the literature (PMID: 18451245; 11698418; 25312647), with the bands greater than 37 kDa predicted to be glycosylated SLAMF7/CS1.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230945).

  • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling SLAMF7/CS1 with ab230945 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly cytoplasmic staining in plasma cells of human colon (PMID: 24299175) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230945).

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling SLAMF7/CS1 with ab230945 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly cytoplasmic staining in plasma cells of human tonsil is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230945).

  • Immunohistochemical analysis of paraffin-embedded human multiple myeloma tissue labeling SLAMF7/CS1 with ab230945 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly cytoplasmic staining in human multiple myeloma (PMID: 26005365; PMID: 18451245) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230945).

References

ab233090 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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