Recombinant
RabMAb

Recombinant Anti-SLAMF7/CS1 antibody [EPR22948-114] (ab223201)

Overview

  • Product name

    Anti-SLAMF7/CS1 antibody [EPR22948-114]
    See all SLAMF7/CS1 primary antibodies
  • Description

    Rabbit monoclonal [EPR22948-114] to SLAMF7/CS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P,IP or WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human SLAMF7/CS1 aa 1-250. The exact sequence is proprietary.
    Database link: Q9NQ25-1

  • Positive control

    • Flow Cyt: Human PBMCs.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22948-114
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P,IP or WB.
  • Target

    • Relevance

      SLAMF7 contains one Ig-like C2-type (immunoglobulin-like) domain. Isoform 1 mediates NK cell activation through a SAP-independent extracellular signal-regulated ERK-mediated pathway. It may play a role in lymphocyte adhesion. Isoform 3 does not mediate any activation. SAP can bind the cytoplasmic tail of isoform 1 when phosphorylated in the presence of Fyn (in vitro). SLAMF7 is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Expression was detected in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-, or T-cell lines. The isoform 3 is expressed at much lower level than isoform 1. There are three named isoforms.
    • Cellular localization

      Membrane; Single-pass type I membrane protein.
    • Database links

    • Alternative names

      • 19A antibody
      • 19A24 protein antibody
      • CD 319 antibody
      • CD2 like receptor activating cytotoxic cells antibody
      • CD2 subset 1 antibody
      • CD319 antibody
      • CD319 antigen antibody
      • CRACC antibody
      • CS1 antibody
      • Membrane protein FOAP 12 antibody
      • Novel Ly9 antibody
      • Novel LY9 (lymphocyte antigen 9) like protein antibody
      • Protein 19A antibody
      • SLAM family member 7 antibody
      • SLAMF 7 antibody
      see all

    Images

    • Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling SLAMF7/CS1 with ab223201 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.

      Cells were stained with rabbit IgG (Left) or ab223201 (Right). Then stained with anti-CD3 conjugated to Alexa Fluor® 647 and anti-CD56 conjugated to BV421. Gated on viable CD3(-) cells.

    • Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling SLAMF7/CS1 with ab223201 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.

      Cells were stained with rabbit IgG (Left) or ab223201 (Right). Then stained with anti-CD3 conjugated to Alexa Fluor® 647 and anti-CD56 conjugated to BV421. Gated on viable CD56(-) cells.

    References

    ab223201 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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