• Product name
  • Description
    Rabbit polyclonal to SLBP
  • Host species
  • Specificity
    This antibody reacts with SLBP.
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • Immunogen

    A region within synthetic peptide: INYGKNTIAY DRYIKEVPRH LRQPGIHPKT PNKFKKYSRR SWDQQIKLWK, corresponding to amino acids 142-191 of Human SLBP (Peptide available as ab108380.)

  • Positive control
    • HepG2 cell lysate.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab56027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/312500.
IHC-P 1/100.
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).Can be blocked with SLBP peptide (ab108380). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function
    RNA-binding protein involved in the histone pre-mRNA processing. Binds the stem-loop structure of replication-dependent histone pre-mRNAs and contributes to efficient 3'-end processing by stabilizing the complex between histone pre-mRNA and U7 small nuclear ribonucleoprotein (snRNP), via the histone downstream element (HDE). Plays an important role in targeting mature histone mRNA from the nucleus to the cytoplasm and to the translation machinery. Stabilizes mature histone mRNA and could be involved in cell-cycle regulation of histone gene expression. Involved in the mechanism by which growing oocytes accumulate histone proteins that support early embryogenesis. Binds to the 5' side of the stem-loop structure of histone pre-mRNAs.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the SLBP family.
  • Developmental stage
    Regulated during the cell cycle: protein levels increase 10 to 20 fold in the late G1 and decrease at the S/G2 border.
  • Domain
    Amino acids 31-34, 96-99 and 241-244 are necessary for interaction with the Importin alpha/Importin beta receptor. The first 18 amino acids, amino acids 69-76 and 179-182 are necessary for interaction with TNPO3. Amino acids 31-34, 96-99 and 241-244 are necessary for nuclear localization.
  • Post-translational
    Phosphorylated on Thr-61 and Thr-62 in the S-phase. Phosphorylation of Thr-62 by CDK1 primes phosphorylation of Thr-61 by CK2. Phosphorylation of Thr-62 is required for its degradation by the proteasome at the end of the S phase. Its degradation is not required for histone mRNA degradation at the end of the S phase. All the phosphorylated forms detected are present in the cytoplasm. Both unphosphorylated and phosphorylated forms bind the stem-loop structure of histone mRNAs.
  • Cellular localization
    Cytoplasm. Nucleus. Polyribosome-associated. Localizes predominantly in the nucleus at the G1/G2 phases and the beginning of S phase. Through the S phase, partially redistributes to the cytoplasm. Binding to histone mRNA is necessary for cytoplasmic localization. Shuttles between the nucleus and the cytoplasm. Imported in the nucleus by the Importin alpha/Importin beta receptor.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Hairpin binding protein histone antibody
    • HBP antibody
    • Histone binding protein antibody
    • Histone RNA hairpin binding protein antibody
    • Histone RNA hairpin-binding protein antibody
    • Histone stem-loop-binding protein antibody
    • SLBP antibody
    • SLBP_HUMAN antibody
    • Stem loop (histone) binding protein antibody
    • Stem loop binding protein antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling SLBP with ab56027 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as teh secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - SLBP. Right - Merge.
  • Anti-SLBP antibody (ab56027) at 0.25 µg/ml + HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa


ab56027 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

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Would you also please complete the Western blot form sent to you previously? In case you did not get it, I will attach the Word the document again.

Could you confirm what the lysyis buffer contained?
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I have received the Western blot images, thank you very much. However, the completed Questionnaire have not come through yet. Would you be so kind to provide some further information about the Western blot procedu...

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As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happil...

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I am happy to take a look at the protocol if you wish to share some details with me. Even if the Abpromise may have been expired.

Let me know if I can help any further.

I am sorry to hear that you have been experiencing problems using ab56027 in the application that you wish.

These two antibodies (ab56027 and ab56329) are from different sources and as their datasheets indicate there are differences i.e. clon...

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I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to ar...

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