Overview

  • Product name
  • Description
    Rabbit polyclonal to SLC10A1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human SLC10A1 aa 141-154 (internal sequence).
    Sequence:

    YSRGIYDGDLKDKV


    Database link: Q14973

  • Positive control
    • IHC-P: Human liver FFPE tissue sections; Human liver cancer tissue. WB: Mouse and rat liver lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab131084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 38 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/50.

Target

  • Function
    The hepatic sodium/bile acid uptake system exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. It is strictly dependent on the extracellular presence of sodium.
  • Sequence similarities
    Belongs to the sodium:bile acid symporter family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell growth-inhibiting gene 29 protein antibody
    • GIG29 antibody
    • Growth inhibiting protein 29 antibody
    • Na / bile acid cotransporter antibody
    • Na / taurocholate transport protein antibody
    • Na(+)/bile acid cotransporter antibody
    • Na(+)/taurocholate transport protein antibody
    • Na/taurocholate cotransporting polypeptide antibody
    • NTCP antibody
    • NTCP_HUMAN antibody
    • NTCP1 antibody
    • SLC10A1 antibody
    • Sodium/bile acid cotransporter antibody
    • Sodium/taurocholate cotransporter polypeptide, hepatic antibody
    • Sodium/taurocholate cotransporting polypeptide antibody
    • Solute carrier family 10 (sodium/bile acid cotransporter family) member 1 antibody
    • Solute carrier family 10 member 1 antibody
    see all

Images

  • All lanes : Anti-SLC10A1 antibody (ab131084) at 0.5 µg

    Lane 1 : Rat liver lysate
    Lane 2 : Mouse liver lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 38 kDa

  • Paraffin-embedded human liver cancer tissue stained for SLC10A1 using ab131084 at 1 µg/ml in immunohistochemical analysis.

  • Immunohistochemical analysis of (1:1) methanol-acetone-fixed human liver tissue, labelling SLC10A1 with ab131084 at a concentration of 1/50, incubated for 16 hours at 4°C. Blocking was performed with BSA at 10% for 1 hour at 20°C. The secondary used was a Goat anti-Rabbit polyclonal Alexa Fluor® 568 conjugate at a concentration of 1/400. The green counterstain is against F-actin using a phalloidin conjugate incubated at a 1/200 dilution for 20 minutes.

    See Abreview

  • IHC image of SLC10A1 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab131084, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Pewkliang Y  et al. A novel immortalized hepatocyte-like cell line (imHC) supports in vitro liver stage development of the human malarial parasite Plasmodium vivax. Malar J 17:50 (2018). Read more (PubMed: 29370800) »
  • Bu P  et al. Berberine-induced Inactivation of Signal Transducer and Activator of Transcription 5 Signaling Promotes Male-specific Expression of a Bile Acid Uptake Transporter. J Biol Chem 292:4602-4613 (2017). Read more (PubMed: 28154180) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (human liver)
Permeabilization
No
Specification
human liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
methanol:acetone (ratio 1:1)

Abcam user community

Verified customer

Submitted Oct 30 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Cryopreserved human hepatocytes)
Permeabilization
Yes - Triton X-100 0.1%
Specification
Cryopreserved human hepatocytes
Blocking step
Serum as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde

Melina Malinen

Verified customer

Submitted Sep 22 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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