Anti-SLC10A1/NTCP1 antibody (ab131084)
Key features and details
- Rabbit polyclonal to SLC10A1/NTCP1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SLC10A1/NTCP1 antibody
See all SLC10A1/NTCP1 primary antibodies -
Description
Rabbit polyclonal to SLC10A1/NTCP1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human SLC10A1/NTCP1 aa 141-154 (internal sequence).
Sequence:YSRGIYDGDLKDKV
Database link: Q14973 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
Use at an assay dependent concentration.
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WB | (3) |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 38 kDa.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 38 kDa. |
Target
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Function
The hepatic sodium/bile acid uptake system exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. It is strictly dependent on the extracellular presence of sodium. -
Sequence similarities
Belongs to the sodium:bile acid symporter family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6554 Human
- Entrez Gene: 20493 Mouse
- Entrez Gene: 24777 Rat
- Omim: 182396 Human
- SwissProt: Q14973 Human
- SwissProt: O08705 Mouse
- SwissProt: P26435 Rat
- Unigene: 952 Human
see all -
Alternative names
- Cell growth-inhibiting gene 29 protein antibody
- GIG29 antibody
- Growth inhibiting protein 29 antibody
see all
Images
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All lanes : Anti-SLC10A1/NTCP1 antibody (ab131084) at 0.5 µg
Lane 1 : Rat liver lysate
Lane 2 : Mouse liver lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 38 kDa -
Paraffin-embedded human liver cancer tissue stained for SLC10A1/NTCP1 using ab131084 at 1 µg/ml in immunohistochemical analysis.
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IHC image of SLC10A1/NTCP1 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab131084, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab131084 has been referenced in 18 publications.
- Yang Y et al. Human menstrual blood-derived stem cell transplantation suppresses liver injury in DDC-induced chronic cholestasis. Stem Cell Res Ther 13:57 (2022). PubMed: 35123555
- Li JY et al. New and effective method to develop primary hepatocytes from liver cancer patients. Exp Biol Med (Maywood) 247:972-981 (2022). PubMed: 35470702
- Komatsu H et al. Signature of chronic hepatitis B virus infection in nails and hair. BMC Infect Dis 22:431 (2022). PubMed: 35509029
- Wang W et al. Transcriptome and Gut Microbiota Profiling Analysis of ANIT-Induced Cholestasis and the Effects of Da-Huang-Xiao-Shi Decoction Intervention. Microbiol Spectr 10:e0324222 (2022). PubMed: 36409145
- Zheng Z et al. A High Hepatic Uptake of Conjugated Bile Acids Promotes Colorectal Cancer-Associated Liver Metastasis. Cells 11:N/A (2022). PubMed: 36497071