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Our Abpromise guarantee covers the use of ab131084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 38 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Paraffin-embedded human liver cancer tissue stained for SLC10A1 using ab131084 at 1 µg/ml in immunohistochemical analysis.
Immunohistochemical analysis of (1:1) methanol-acetone-fixed human liver tissue, labelling SLC10A1 with ab131084 at a concentration of 1/50, incubated for 16 hours at 4°C. Blocking was performed with BSA at 10% for 1 hour at 20°C. The secondary used was a Goat anti-Rabbit polyclonal Alexa Fluor® 568 conjugate at a concentration of 1/400. The green counterstain is against F-actin using a phalloidin conjugate incubated at a 1/200 dilution for 20 minutes.
IHC image of SLC10A1 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab131084, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"