The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 38 kDa.
1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
The hepatic sodium/bile acid uptake system exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. It is strictly dependent on the extracellular presence of sodium.
Flow cytometric analysis of K562 cells (right histogram) labeling SLC10A1 with ab175289 at 1/10 dilution compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot - Anti-SLC10A1 antibody - C-terminal (ab175289)
Anti-SLC10A1 antibody - C-terminal (ab175289) at 1/1000 dilution + K562 cell line lysate at 15 µg