Product nameAnti-SLC10A1/NTCP1 antibody
See all SLC10A1/NTCP1 primary antibodies
DescriptionRabbit polyclonal to SLC10A1/NTCP1
Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- IHC-P: Human liver FFPE tissue sections; Human liver cancer tissue. WB: Mouse and rat liver lysates.
This product was previously labelled as SLC10A1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab131084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 38 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionThe hepatic sodium/bile acid uptake system exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. It is strictly dependent on the extracellular presence of sodium.
Sequence similaritiesBelongs to the sodium:bile acid symporter family.
- Information by UniProt
- Cell growth-inhibiting gene 29 protein antibody
- GIG29 antibody
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All lanes : Anti-SLC10A1/NTCP1 antibody (ab131084) at 0.5 µg
Lane 1 : Rat liver lysate
Lane 2 : Mouse liver lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 38 kDa
Paraffin-embedded human liver cancer tissue stained for SLC10A1/NTCP1 using ab131084 at 1 µg/ml in immunohistochemical analysis.
Immunohistochemical analysis of (1:1) methanol-acetone-fixed human liver tissue, labelling SLC10A1/NTCP1 with ab131084 at a concentration of 1/50, incubated for 16 hours at 4°C. Blocking was performed with BSA at 10% for 1 hour at 20°C. The secondary used was a Goat anti-Rabbit polyclonal Alexa Fluor® 568 conjugate at a concentration of 1/400. The green counterstain is against F-actin using a phalloidin conjugate incubated at a 1/200 dilution for 20 minutes.
IHC image of SLC10A1/NTCP1 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab131084, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab131084 has been referenced in 5 publications.
- Malinen MM et al. Protein expression and function of organic anion transporters in short-term and long-term cultures of Huh7 human hepatoma cells. Eur J Pharm Sci 130:186-195 (2019). PubMed: 30685239
- Chen YS et al. Ursodeoxycholic Acid Regulates Hepatic Energy Homeostasis and White Adipose Tissue Macrophages Polarization in Leptin-Deficiency Obese Mice. Cells 8:N/A (2019). PubMed: 30884843
- Pewkliang Y et al. A novel immortalized hepatocyte-like cell line (imHC) supports in vitro liver stage development of the human malarial parasite Plasmodium vivax. Malar J 17:50 (2018). PubMed: 29370800
- Bu P et al. Berberine-induced Inactivation of Signal Transducer and Activator of Transcription 5 Signaling Promotes Male-specific Expression of a Bile Acid Uptake Transporter. J Biol Chem 292:4602-4613 (2017). PubMed: 28154180
- Liu L et al. Hepatic Tmem30a Deficiency Causes Intrahepatic Cholestasis by Impairing Expression and Localization of Bile Salt Transporters. Am J Pathol 187:2775-2787 (2017). PubMed: 28919113